Diamond squid (Thysanoteuthis rhombus)-derived chondroitin sulfate stimulates bone healing within a rat calvarial defect.
Diamond squid (Thysanoteuthis rhombus)-derived chondroitin sulfate stimulates bone healing within a rat calvarial defect.
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DOI：
10.3390/md11125024
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发表时间：
2013-12-11
期刊：
影响因子：
5.4
通讯作者：
Uehara M
中科院分区：
文献类型：
--
作者：
Hosaka YZ;Iwai Y;Tamura J;Uehara M
硫酸软骨素（CS）被认为参与骨形成和矿化过程。先前的研究表明，鱿鱼源性CS（sqCS）在体外与骨形态发生蛋白（BMP）-4配合具有成骨能力。然而，在体内，成骨潜力尚未得到验证。在这项研究中，我们在大鼠颅骨中制造了一个临界尺寸的骨缺损，并将负载 sqCS 的明胶水凝胶海绵 (Gel) 植入有或没有 BMP-4 的缺损中（分别为 CS/BMP/Gel 和 CS/Gel）。 15周时，CS/Gel处理的缺损和CS/BMP/Gel处理的缺损的骨修复率分别为47.2%和51.1%，而空缺损和未经处理的海绵缺损显示出明显较少的骨长入。再生骨的 von Kossa 染色强度低于原始骨。所有治疗组之间第 9 至 10 周的矿物质沉积率没有显着差异。尽管骨修复尚未完成，但 sqCS 在没有 BMP-4 且没有外部间充质细胞或前成骨细胞的情况下刺激了骨再生。因此，sqCS是一种有前途的促进成骨的物质。
Chondroitin sulfate (CS) has been suggested to be involved in bone formation and mineralization processes. A previous study showed that squid-derived CS (sqCS) has osteoblastogenesis ability in cooperation with bone morphogenetic protein (BMP)-4 in vitro. However, in vivo, osteogenic potential has not been verified. In this study, we created a critical-sized bone defect in the rat calvaria and implanted sqCS-loaded gelatin hydrogel sponges (Gel) into the defect with or without BMP-4 (CS/BMP/Gel and CS/Gel, respectively). At 15 weeks, bone repair rate of CS/Gel-treated defects and CS/BMP/Gel-treated defects were 47.2% and 51.1%, respectively, whereas empty defects and defects with untreated sponges showed significantly less bone ingrowth. The intensity of von Kossa staining of the regenerated bone was less than that of the original one. Mineral apposition rates at 9 to 10 weeks were not significantly different between all treatment groups. Although bone repair was not completed, sqCS stimulated bone regeneration without BMP-4 and without external mesenchymal cells or preosteoblasts. Therefore, sqCS is a promising substance for promotion of osteogenesis.
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DOI：
10.1016/j.matbio.2008.11.003
发表时间：
2009-01
期刊：
Matrix biology : journal of the International Society for Matrix Biology
影响因子：
--
作者：
Mochida Y;Parisuthiman D;Pornprasertsuk-Damrongsri S;Atsawasuwan P;Sricholpech M;Boskey AL;Yamauchi M
通讯作者：
Yamauchi M
影响因子：
2.8
作者：
McCullough, Kirk A.;Waits, Chad A.;Anderson, H. Clarke
通讯作者：
Anderson, H. Clarke
影响因子：
14
作者：
Sawyer, A. A.;Song, S. J.;Cool, S. M.
通讯作者：
Cool, S. M.
影响因子：
14
作者：
Lee, Young Jun;Lee, Ji-Hye;Shin, Heungsoo
通讯作者：
Shin, Heungsoo
影响因子：
4.2
作者：
Boskey, AL;Spevak, L;Rosenberg, L
通讯作者：
Rosenberg, L