Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1-to-M2 transformation.
Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1-to-M2 transformation.
复制标题
人脐带间充质干细胞通过促进M1至M2转化来减轻细胞外囊泡，从而减轻盐酸炎。
DOI：
10.3389/fphys.2023.1131701
复制
发表时间：
2023
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
作者：
背景：随着沙眼衣原体(CT)感染后慢性输卵管炎致不孕症患者的增多，对组织修复或再生治疗的需求也越来越迫切。人脐带间充质干细胞来源的细胞外小泡(hucMSC-EV)治疗提供了一种有吸引力的无细胞治疗方法。方法：通过动物体内实验，观察hucMSC-EV对沙眼衣原体所致输卵管炎性不孕的缓解作用。此外，我们还检测了hucMSC-EV诱导巨噬细胞极化的作用，以探讨其分子机制。结果：hucMSC-EV治疗组沙眼衣原体感染所致的输卵管炎性不孕较对照组明显减轻。进一步的机制实验表明，hucMSC-EV通过NF-κB信号通路诱导巨噬细胞从M1向M2极化，改善了输卵管局部炎症微环境，抑制了输卵管炎症。结论：我们得出结论，这种方法代表了一种有希望的无细胞途径来改善慢性输卵管炎所致的不孕症。
Background: With an increasing number of patients experiencing infertility due to chronic salpingitis after Chlamydia trachomatis (CT) infection, there is an unmet need for tissue repair or regeneration therapies. Treatment with human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hucMSC-EV) provides an attractive cell-free therapeutic approach. Methods: In this study, we investigated the alleviating effect of hucMSC-EV on tubal inflammatory infertility caused by CT using in vivo animal experiments. Furthermore, we examined the effect of hucMSC-EV on inducing macrophage polarization to explore the molecular mechanism. Results: Our results showed that tubal inflammatory infertility caused by Chlamydia infection was significantly alleviated in the hucMSC-EV treatment group compared with the control group. Further mechanistic experiments showed that the application of hucMSC-EV induced macrophage polarization from the M1 to the M2 type via the NF-κB signaling pathway, improved the local inflammatory microenvironment of fallopian tubes and inhibited tube inflammation. Conclusion: We conclude that this approach represents a promising cell-free avenue to ameliorate infertility due to chronic salpingitis.
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DOI：
10.1002/stem.3380
发表时间：
2021-09
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Galipeau J
通讯作者：
Galipeau J
影响因子：
16
作者：
Lee, In Young;Lim, Jane Melissa;Choi, Eui-Ju
通讯作者：
Choi, Eui-Ju
影响因子：
4.8
作者：
Leibowitz SM;Yan J
通讯作者：
Yan J
影响因子：
5.6
作者：
Korbecki J;Simińska D;Gąssowska-Dobrowolska M;Listos J;Gutowska I;Chlubek D;Baranowska-Bosiacka I
通讯作者：
Baranowska-Bosiacka I
影响因子：
7.3
作者：
Barnabei L;Laplantine E;Mbongo W;Rieux-Laucat F;Weil R
通讯作者：
Weil R