Why is the partial oxygen pressure of human tissues a crucial parameter? Small molecules and hypoxia.
Why is the partial oxygen pressure of human tissues a crucial parameter? Small molecules and hypoxia.
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DOI：
10.1111/j.1582-4934.2011.01258.x
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发表时间：
2011-06
影响因子：
5.3
通讯作者：
Kieda C
中科院分区：
文献类型：
--
作者：
Carreau A;El Hafny-Rahbi B;Matejuk A;Grillon C;Kieda C
氧气的供应和扩散进入组织是生存所必需的。氧分压(PO2)是器官生理状态的重要组成部分，是氧供需平衡的结果。在哺乳动物中，氧气是由组织良好的血管系统中循环的红细胞运输的。氧气的输送取决于每个器官的代谢需求和功能状态。因此，在生理条件下，器官和组织具有其独特的“组织常氧”或“生理氧合”状态。在癌症、糖尿病、冠心病、中风等与PO2降低相关的病理情况下，组织氧合会受到严重干扰，即‘缺氧’。在这篇综述中，我们介绍了一系列目前用于评估组织氧合的方法。我们的研究表明，低氧在肿瘤的发展过程中是显著的，并且对氧合及其对化疗效率的影响有很强的后果。然后我们将其与人体器官的生理PO2值进行比较。最后，我们评估了高压氧对细胞活性及其分子调控的影响。更重要的是，我们强调体内和体外组织和细胞氧状态的差异，这可能会对实验结果产生不利影响。与生理氧合相对应的数值似乎在11%到1%O2之间，而目前的体外实验通常是在19.95%O2中进行的，就氧平衡而言，这是一种人工环境。重要的是要认识到，在所谓的常氧条件下进行的大多数实验可能具有危险的误导性。
Oxygen supply and diffusion into tissues are necessary for survival. The oxygen partial pressure (pO2), which is a key component of the physiological state of an organ, results from the balance between oxygen delivery and its consumption. In mammals, oxygen is transported by red blood cells circulating in a well-organized vasculature. Oxygen delivery is dependent on the metabolic requirements and functional status of each organ. Consequently, in a physiological condition, organ and tissue are characterized by their own unique ‘tissue normoxia’ or ‘physioxia’ status. Tissue oxygenation is severely disturbed during pathological conditions such as cancer, diabetes, coronary heart disease, stroke, etc., which are associated with decrease in pO2, i.e. ‘hypoxia’. In this review, we present an array of methods currently used for assessing tissue oxygenation. We show that hypoxia is marked during tumour development and has strong consequences for oxygenation and its influence upon chemotherapy efficiency. Then we compare this to physiological pO2 values of human organs. Finally we evaluate consequences of physioxia on cell activity and its molecular modulations. More importantly we emphasize the discrepancy between in vivo and in vitro tissue and cells oxygen status which can have detrimental effects on experimental outcome. It appears that the values corresponding to the physioxia are ranging between 11% and 1% O2 whereas current in vitro experimentations are usually performed in 19.95% O2, an artificial context as far as oxygen balance is concerned. It is important to realize that most of the experiments performed in so-called normoxia might be dangerously misleading.
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影响因子：
2.7
作者：
Fylaktakidou, KC;Lehn, JM;Nicolau, C
通讯作者：
Nicolau, C
影响因子：
158.5
作者：
GLUCKMAN, E;BROXMEYER, HE;BOYSE, EA
通讯作者：
BOYSE, EA
DOI：
10.1046/j.1365-201x.2000.00713.x
发表时间：
2000-04-01
期刊：
ACTA PHYSIOLOGICA SCANDINAVICA
影响因子：
--
作者：
Boushel, R;Piantadosi, CA
通讯作者：
Piantadosi, CA
抗血管剂5,6-二甲基苯乙烯-4-乙酸诱导内皮细胞凋亡。
影响因子：
8.8
作者：
Ching, LM;Cao, Z;Kieda, C;Zwain, S;Jameson, MB;Baguley, BC
通讯作者：
Baguley, BC
DOI：
10.1034/j.1600-0838.2001.110404.x
发表时间：
2001-08-01
影响因子：
4.1
作者：
Boushel, R;Langberg, H;Kjær, M
通讯作者：
Kjær, M