Probiotic use decreases intestinal inflammation and increases bone density in healthy male but not female mice.
Probiotic use decreases intestinal inflammation and increases bone density in healthy male but not female mice.
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DOI：
10.1002/jcp.24340
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发表时间：
2013-08
影响因子：
5.6
通讯作者：
Britton, Robert A.
中科院分区：
文献类型：
--
作者：
Mccabe, Laura R.;Irwin, Regina;Schaefer, Laura;Britton, Robert A.
骨质疏松症可由肠道炎症引起，如炎症性肠病所见。益生菌是一种微生物，当摄入足够的量时，对宿主提供健康益处，可以具有抗炎特性，目前正在研究治疗炎症性肠病。在这里，我们研究了用罗伊氏乳杆菌ATCC PTA 6475（一种具有抗TNF α活性的候选益生菌）治疗健康雄性小鼠是否会影响肠道TNFα水平并增强骨密度。成年雄性小鼠给予L. reuteri 6475，经口灌胃给药3 ×/周，持续4周。空肠和回肠RNA图谱的检查表明L。reuteri抑制雄性小鼠空肠和回肠中的基础TNFα mRNA水平，但令人惊讶的是，在雌性小鼠中没有。接下来，我们检查了骨反应。显微CT显示L. Reuteri 6475治疗增加了远端股骨干骺端区域以及腰椎中的男性骨小梁参数（矿物质密度、骨体积分数、骨小梁数量和骨小梁厚度）。皮质骨参数未受影响。动态和静态组织形态计量学和血清重构参数表明，L。罗伊氏摄入增加雄性小鼠成骨细胞血清标记物和骨形成的动态测量。与雄性小鼠相比，L. reuteri对雌性小鼠的骨参数没有影响。总之，我们的研究表明，股骨和脊椎骨形成增加，以响应口服益生菌的使用，导致增加骨小梁体积在雄性小鼠。
Osteoporosis can result from intestinal inflammation, as is seen with inflammatory bowel disease. Probiotics, microorganisms that provide a health benefit to the host when ingested in adequate amounts, can have anti-inflammatory properties and are currently being examined to treat inflammatory bowel disease. Here, we examined if treating healthy male mice with Lactobacillus reuteri ATCC PTA 6475 (a candidate probiotic with anti-TNFα activity) could affect intestinal TNFα levels and enhance bone density. Adult male mice were given L. reuteri 6475 orally by gavage for 3 ×/week for 4 weeks. Examination of jejunal and ileal RNA profiles indicates that L. reuteri suppressed basal TNFα mRNA levels in the jejunum and ileum in male mice, but surprisingly not in female mice. Next, we examined bone responses. Micro-computed tomography demonstrated that L. reuteri 6475 treatment increased male trabecular bone parameters (mineral density, bone volume fraction, trabecular number, and trabecular thickness) in the distal femur metaphyseal region as well as in the lumbar vertebrae. Cortical bone parameters were unaffected. Dynamic and static histomorphometry and serum remodeling parameters indicate that L. reuteri ingestion increases osteoblast serum markers and dynamic measures of bone formation in male mice. In contrast to male mice, L. reuteri had no effect on bone parameters in female mice. Taken together our studies indicate that femoral and vertebral bone formation increases in response to oral probiotic use, leading to increased trabecular bone volume in male mice.
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发表时间：
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作者：
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