Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials.
Mesenchymal stem cells derived from human iPS cells via mesoderm and neuroepithelium have different features and therapeutic potentials.
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DOI：
10.1371/journal.pone.0200790
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Era T
中科院分区：
文献类型：
--
作者：
Eto S;Goto M;Soga M;Kaneko Y;Uehara Y;Mizuta H;Era T
从成人组织中分离的间充质干细胞（MSC）能够在体外增殖并保持其多能性，使其成为再生医学的有吸引力的细胞来源。然而，在目前的准备制度下，自我更新的可用性和能力是有限的。诱导多能干细胞（iPSC）现在提供了一种替代的、与MSC相似的细胞来源。在此，我们建立了通过中胚层样和神经上皮样细胞将hiPSC分化为MSC的新方法。两种来源的MSC群体都表现出自我更新和多能性，以及在皮肤伤口、压疮和骨关节炎小鼠模型中的治疗潜力。有趣的是，在疾病模型中两种类型的MSC之间的治疗效果不同，这表明治疗效果取决于细胞来源。我们的研究结果为此类细胞的临床应用提供了有价值的基本见解。
Mesenchymal stem cells (MSCs) isolated from adult human tissues are capable of proliferating in vitro and maintaining their multipotency, making them attractive cell sources for regenerative medicine. However, the availability and capability of self-renewal under current preparation regimes are limited. Induced pluripotent stem cells (iPSCs) now offer an alternative, similar cell source to MSCs. Herein, we established new methods for differentiating hiPSCs into MSCs via mesoderm-like and neuroepithelium-like cells. Both derived MSC populations exhibited self-renewal and multipotency, as well as therapeutic potential in mouse models of skin wounds, pressure ulcers, and osteoarthritis. Interestingly, the therapeutic effects differ between the two types of MSCs in the disease models, suggesting that the therapeutic effect depends on the cell origin. Our results provide valuable basic insights for the clinical application of such cells.
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影响因子：
23.9
作者：
Caplan AI;Correa D
通讯作者：
Correa D
影响因子：
3.7
作者：
Fukuta M;Nakai Y;Kirino K;Nakagawa M;Sekiguchi K;Nagata S;Matsumoto Y;Yamamoto T;Umeda K;Heike T;Okumura N;Koizumi N;Sato T;Nakahata T;Saito M;Otsuka T;Kinoshita S;Ueno M;Ikeya M;Toguchida J
通讯作者：
Toguchida J
影响因子：
2.1
作者：
Okada, Seiji;Harada, Hideki;Suzu, Shinya
通讯作者：
Suzu, Shinya
影响因子：
7
作者：
Christiansen BA;Guilak F;Lockwood KA;Olson SA;Pitsillides AA;Sandell LJ;Silva MJ;van der Meulen MC;Haudenschild DR
通讯作者：
Haudenschild DR
影响因子：
4.6
作者：
Kasuya A;Sakabe J;Tokura Y
通讯作者：
Tokura Y