Potential application of in vivo imaging of impaired lymphatic duct to evaluate the severity of pressure ulcer in mouse model.
Potential application of in vivo imaging of impaired lymphatic duct to evaluate the severity of pressure ulcer in mouse model.
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DOI：
10.1038/srep04173
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发表时间：
2014-02-25
影响因子：
4.6
通讯作者：
Tokura Y
中科院分区：
文献类型：
--
作者：
Kasuya A;Sakabe J;Tokura Y
缺血再灌注（IR）损伤是压疮的一个原因。然而，IR损伤引起的淋巴管损伤的机制仍不清楚。我们研究了小鼠皮肤 IR 模型中淋巴管结构和功能的变化。我们提出了一种评估压疮严重程度的新方法。免疫组织化学显示，IR损伤后淋巴管完全消失，而血管相对保存。受伤组织中有害活性氧（ROS）的产生增加。体外研究表明淋巴内皮细胞对 ROS 高度脆弱。然后，我们使用皮内注射吲哚菁绿（ICG）的体内成像系统评估受损的淋巴引流。 ICG引流功能障碍与随后皮肤变化的严重程度呈正相关。通过该成像系统对淋巴管功能障碍进行量化可能是评估压疮严重程度的有用策略。
Ischemia-reperfusion (IR) injury is a cause of pressure ulcer. However, a mechanism underlying the IR injury-induced lymphatic vessel damage remains unclear. We investigated the alterations of structure and function of lymphatic ducts in a mouse cutaneous IR model. And we suggested a new method for evaluating the severity of pressure ulcer. Immunohistochemistry showed that lymphatic ducts were totally vanished by IR injury, while blood vessels were relatively preserved. The production of harmful reactive oxygen species (ROS) was increased in injured tissue. In vitro study showed a high vulnerability of lymphatic endothelial cells to ROS. Then we evaluated the impaired lymphatic drainage using an in vivo imaging system for intradermally injected indocyanine green (ICG). The dysfunction of ICG drainage positively correlated with the severity of subsequent cutaneous changes. Quantification of the lymphatic duct dysfunction by this imaging system could be a useful strategy to estimate the severity of pressure ulcer.
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影响因子：
3.8
作者：
Loerakker, S.;Stekelenburg, A.;Strijkers, G. J.;Rijpkema, J. J. M.;Baaijens, F. P. T.;Bader, D. L.;Nicolay, K.;Oomens, C. W. J.
通讯作者：
Oomens, C. W. J.
DOI：
10.1016/j.bbrc.2009.09.015
发表时间：
2009-12-25
影响因子：
3.1
作者：
Kikuchi, Kiyoshi;Tancharoen, Salunya;Kawahara, Ko-ichi
通讯作者：
Kawahara, Ko-ichi
DOI：
10.3109/10739689509146769
发表时间：
1995-01-01
期刊：
Microcirculation (New York)
影响因子：
--
作者：
Kubes, Pal
通讯作者：
Kubes, Pal
影响因子：
3.9
作者：
Wang, W;Vadgama, P
通讯作者：
Vadgama, P
影响因子：
20.3
作者：
Eltzschig, HK;Thompson, LF;Colgan, SP
通讯作者：
Colgan, SP