Minimization of self-quenching fluorescence on dyes conjugated to biomolecules with multiple labeling sites via asymmetrically charged NIR fluorophores.
Minimization of self-quenching fluorescence on dyes conjugated to biomolecules with multiple labeling sites via asymmetrically charged NIR fluorophores.
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DOI：
10.1002/cmmi.1585
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发表时间：
2014-09
影响因子：
--
通讯作者：
Berezin, Mikhail Y.
中科院分区：
文献类型：
--
作者：
Zhegalova, Natalia G.;He, Shawn;Zhou, Haiying;Kim, David M.;Berezin, Mikhail Y.
即使在低浓度下，染料的自聚集对制备足够明亮的分子探针以用于活体成像，特别是在近红外菁染料与具有多个标记位点的多肽的结合方面构成了相当大的挑战。这种自聚集导致染料之间的显著能量转移，导致目标探针的严重猝灭和低亮度。为了解决这个问题，我们设计了一种新型的具有不对称电荷分布的染料。不对称的分布防止了生色团的π堆积，从而最大限度地减少了能量转移和荧光猝灭。染料与多肽的结合表明，在吸收光谱中只有很小的H-聚集带，因此具有相对较高的量子效率。
Self-aggregation of dyes even at low concentrations pose a considerable challenge in preparing sufficiently bright molecular probes for in vivo imaging, particularly in the conjugation of near infrared cyanine dyes to polypeptides with multiple labeling sites. Such self-aggregation leads to a significant energy transfer between the dyes resulting in severe quenching and low brightness of the targeted probe. To address this problem, we designed a novel type of dye with an asymmetrical distribution of charge. Asymmetrical distribution prevents the chromophores from π-stacking thus minimizing the energy transfer and fluorescence quenching. The conjugation of the dye to polypeptides showed only a small presence of an H-aggregate band in the absorption spectra and, hence, a relatively high quantum efficiency.
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影响因子：
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