Correlation of a priori DCE-MRI and (1)H-MRS data with molecular markers in neck nodal metastases: Initial analysis.
Correlation of a priori DCE-MRI and (1)H-MRS data with molecular markers in neck nodal metastases: Initial analysis.
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DOI：
10.1016/j.oraloncology.2012.02.001
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发表时间：
2012-08
期刊：
影响因子：
4.8
通讯作者：
Shukla-Dave, Amita
中科院分区：
文献类型：
--
作者：
Jansen, Jacobus F. A.;Carlson, Diane L.;Lu, Yonggang;Stambuk, Hilda E.;Moreira, Andre L.;Singh, Bhuvanesh;Patel, Snehal G.;Kraus, Dennis H.;Wong, Richard J.;Shaha, Ashok R.;Shah, Jatin P.;Shukla-Dave, Amita
本研究旨在探讨头颈部鳞状细胞癌(HNSCC)患者颈淋巴结转移的非侵袭性、预处理生物成像(动态增强磁共振成像[DCE-MRI]和质子磁共振波谱[1H-MRS])与特异性分子标志物的相关性。对12例手术治疗的颈淋巴结转移瘤患者行DCE-MRI和1H-MRS检查。采用免疫组织化学方法检测肿瘤组织中Ki-67(细胞增殖)、血管内皮生长因子(血管内皮生长因子)、碳酸氢酶、缺氧诱导转录因子α和人乳头瘤病毒的表达。此外，根据H&E染色判断坏死率。采用Spearman相关分析比较DCE-MRI、1H-MRS和分子标志物数据。DCEMRI参数std(Kep)与VEGFIHC表达水平显著相关(Rho=0.81，p=0.0001)。此外，Ki-67的表达水平与性传播疾病和性传播疾病呈负相关(Rho=−0.71，p=0.004；rho=−0.73，p=0.003)。其他DCE-MRI、1H-MRS和IHC值无显著相关性。这一初步研究结果表明，转移性HNSCC的血流不均匀程度似乎与血管生成呈正相关，与增殖呈负相关。这些结果是初步的，是指示性的，而不是描述了患有结节疾病的HNSCC患者的趋势。未来需要对更大的患者群体进行研究，以验证和澄清我们的初步发现。
The aim of the present study is to correlate non-invasive, pretreatment biological imaging (dynamic contrast enhanced-MRI [DCE-MRI] and proton magnetic resonance spectroscopy [1H-MRS]) findings with specific molecular marker data in neck nodal metastases of head and neck squamous cell carcinoma (HNSCC) patients. Pretreatment DCE-MRI and 1H-MRS were performed on neck nodal metastases of 12 patients who underwent surgery. Surgical specimens were analyzed with immunohistochemistry (IHC) assays for: Ki-67 (reflecting cellular proliferation), vascular endothelial growth factor (VEGF) (the “endogenous marker” of tumor vessel growth), carbonic anhydrase (CAIX), hypoxia inducible transcription factor (HIF-1α), and human papillomavirus (HPV). Additionally, necrosis was estimated based on H&E staining. The Spearman correlation was used to compare DCE-MRI, 1H-MRS, and molecular marker data. A significant correlation was observed between DCE-MRI parameter std(kep) and VEGF IHC expression level (rho = 0.81, p = 0.0001). Furthermore, IHC expression levels of Ki-67 inversely correlated with std(Ktrans) and std(ve) (rho = −0.71; p = 0.004, and rho = −0.73; p = 0.003, respectively). Other DCE-MRI, 1H-MRS and IHC values did not show significant correlation. The results of this preliminary study indicate that the level of heterogeneity of perfusion in metastatic HNSCC seems positively correlated with angiogenesis, and inversely correlated with proliferation. These results are preliminary in nature and are indicative, and not definitive, trends portrayed in HNSCC patients with nodal disease. Future studies with larger patient populations need to be carried out to validate and clarify our preliminary findings.
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影响因子：
4.8
作者：
Faratzis, Gregory;Tsiambas, Evangelos;Patsouris, Efstratios
通讯作者：
Patsouris, Efstratios
影响因子：
4.8
作者：
Silva, SD;Agostini, M;Graner, E
通讯作者：
Graner, E
影响因子：
9.8
作者：
Jansen, Jacobus F. A.;Koutcher, Jason A.;Shukla-Dave, Amita
通讯作者：
Shukla-Dave, Amita
DOI：
10.1038/sj.neo.7900037
发表时间：
1999-08-01
期刊：
Neoplasia (New York)
影响因子：
--
作者：
Gillies, Robert J.;Schornack, Paul A.;Raghunand, Natarajan
通讯作者：
Raghunand, Natarajan
DOI：
10.1016/j.ijrobp.2010.11.022
发表时间：
2012-01-01
影响因子：
7
作者：
Jansen, Jacobus F. A.;Schoder, Heiko;Lee, Nancy Y.;Stambuk, Hilda. E.;Wang, Ya;Fury, Matthew G.;Patel, Snehal G.;Pfister, David G.;Shah, Jatin P.;Koutcher, Jason A.;Shukla-Dave, Amita
通讯作者：
Shukla-Dave, Amita