Transcript profiling of Paoenia ostii during artificial chilling induced dormancy release identifies activation of GA pathway and carbohydrate metabolism.
Transcript profiling of Paoenia ostii during artificial chilling induced dormancy release identifies activation of GA pathway and carbohydrate metabolism.
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人工冷冻诱导休眠释放过程中芍药的转录谱分析鉴定了 GA 途径和碳水化合物代谢的激活
DOI：
10.1371/journal.pone.0055297
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zheng G
中科院分区：
文献类型：
--
作者：
Gai S;Zhang Y;Liu C;Zhang Y;Zheng G
内休眠的花蕾必须经过一段时间的低温才能重新生长和开花，这是牡丹冬季促成栽培的主要障碍。利用定制的cDNA微阵列（8×15 K元件）研究了牡丹品种“枫丹白”花蕾在0-4°C低温处理24 d后的基因表达谱。根据整株转入绿色大棚后的形态变化，低温处理18 d后解除了内休眠，延长低温处理时间可提高萌芽率。皮尔逊相关等级聚类的样品组是高度一致的休眠转变所揭示的形态变化。共检测到3,174个差异显著（P<0.05）的基因，其中上调基因1,611个，下调基因1,563个，差异表达基因数基本相同。对差异表达基因的功能注释表明，差异表达基因在细胞过程、代谢过程、对刺激的反应、生物学过程的调控和发育过程中均有较好的表达。系统聚类分析表明，这些基因的激活与糖酵解、柠檬酸循环和戊糖磷酸途径、能量代谢和细胞生长有关。根据GO分析结果，共筛选出51个与GA反应和GA信号通路相关的探针，其中22个探针表达差异较大。表达谱分析还揭示了GA生物合成、信号转导和响应基因参与了内源休眠解除。我们推测GA途径的激活在牡丹休眠解除的调控中起着核心作用。
Endo-dormant flower buds must pass through a period of chilling to reinitiate growth and subsequent flowering, which is a major obstacle to the forcing culture of tree peony in winter. Customized cDNA microarray (8×15 K element) was used to investigate gene expression profiling in tree peony ‘Feng Dan Bai’ buds during 24 d chilling treatment at 0–4°C. According to the morphological changes after the whole plants were transferred to green house, endo-dormancy was released after 18 d chilling treatment, and prolonged chilling treatment increased bud break rate. Pearson correlation hierarchical clustering of sample groups was highly consistent with the dormancy transitions revealed by morphological changes. Totally 3,174 significantly differentially-expressed genes (P<0.05) were observed through endo-dormancy release process, of which the number of up-regulated (1,611) and that of down-regulated (1,563) was almost the same. Functional annotation of differentially-expressed genes revealed that cellular process, metabolic process, response to stimulus, regulation of biological process and development process were well-represented. Hierarchical clustering indicated that activation of genes involved in carbohydrate metabolism (Glycolysis, Citrate cycle and Pentose phosphate pathway), energy metabolism and cell growth. Based on the results of GO analysis, totally 51 probes presented in the microarray were associated with GA response and GA signaling pathway, and 22 of them were differently expressed. The expression profiles also revealed that the genes of GA biosynthesis, signaling and response involved in endo-dormancy release. We hypothesized that activation of GA pathway played a central role in the regulation of dormancy release in tree peony.
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影响因子：
2.6
作者：
Huang, Xin;Xue, Tongtong;Zheng, Guosheng
通讯作者：
Zheng, Guosheng
影响因子：
5.1
作者：
DEVITT, ML;STAFSTROM, JP
通讯作者：
STAFSTROM, JP
影响因子：
7.2
作者：
Gilmour, SJ;Zarka, DG;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
4.3
作者：
Halaly, Tamar;Pang, Xuequn;Or, Etti
通讯作者：
Or, Etti
影响因子：
16
作者：
Hwang, WW;Venkatasubrahmanyam, S;Madhani, HD
通讯作者：
Madhani, HD