Effects of operation conditions on enzymatic hydrolysis of high-solid rice straw

Effects of operation conditions on enzymatic hydrolysis of high-solid rice straw
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操作条件对高固体稻秆酶解的影响

DOI:
10.1016/j.ijhydene.2012.02.080
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发表时间:
2012-09
影响因子:
7.2
通讯作者:
Wang, Yan-Mei
Wang, Yan-Mei
中科院分区:
工程技术2区
文献类型:
--
作者:
Ran, Yao;Wang, Yong-Zhong;Liao, Qiang;Zhu, Xun;Chen, Rong;Lee, Duu-Jong;Wang, Yan-Mei

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本文对水稻秸秆的高固酶解性能进行了实验研究。同时用热重分析仪测定了稻草水解前后的热流和失重情况。研究发现,随着供水量、水解温度和底物网目数的增加,还原糖的产量和产率呈先增加后降低的趋势,而剩余底物中纤维素含量呈相反的趋势。此外,高酶负荷导致纤维素的糖化效率恒定。在供水量1.0ml/(g底物)、酶解温度50℃、稻草秸秆60目、纤维素酶用量2.0mg/(g底物)的最优条件下,糖的产量分别为77.7mg/(g底物)和0.44g/g。同时对稻草进行热重分析表明,酶解后稻草的化学成分发生了变化。
In the present work, the performances of high-solids enzymatic hydrolysis of rice straw were experimentally studied. Moreover, the heat flow and weight loss of the pre- and post-hydrolysis of rice straw were measured by the simultaneous thermogravimetric analyzer. It is found that the output and yield of reducing sugar initially increased and then reduced with an increase of supplied water, hydrolysis temperature and substrate mesh, while the cellulose content in residual substrate had an opposite trends. Furthermore, a high enzyme loading resulted in a constant saccharification efficiency of cellulose. The output and yield of the sugar, 77.7mg/(g substrate) and 0.44g/g, were respectively achieved under the optimal conditions of the supplied water of 1.0ml/(g substrate), enzymolysis temperature of 50°C, rice straw of 60 mesh and the cellulase dosage of 2.0mg/(g substrate). Simultaneous thermogravimetric analysis on the rice straw indicates that the chemical compositions of rice straw changed after enzymolysis.
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