Differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers.
Differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers.
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作者：
Furet JP;Kong LC;Tap J;Poitou C;Basdevant A;Bouillot JL;Mariat D;Corthier G;Doré J;Henegar C;Rizkalla S;Clément K
肥胖改变了肠道微生物群生态，并与人类的低度炎症有关。Roux-en-Y胃旁路术（RYGB）是治疗病态肥胖症的最有效的手术之一，可导致体重急剧减轻并改善代谢和炎症状态。我们分析了RYGB对肠道微生物群改变的影响，并检查了与此过程相关的适应性联系。通过实时定量PCR分析了13名瘦对照受试者和30名肥胖个体（7名2型糖尿病患者）在RYGB之前（M0），3个月（M3）和6个月（M6）的粪便样本中的肠道微生物群。突出显示了四个主要结果：1）M0时肥胖受试者中拟杆菌/普雷沃菌组低于对照受试者，M3时增加。2）大肠杆菌在M3时增加，与脂肪量和瘦素水平呈负相关，与摄食量无关; 3）乳酸菌（包括乳杆菌/明串珠菌/片球菌群和双歧杆菌属）在M3时减少;和4）患有糖尿病的受试者中的普氏粪杆菌种类较低，并且在M0和手术后的整个随访期间与炎症标志物呈负相关，与食物摄入的变化无关。这些结果表明，在RYGB诱导的饥饿样情况下，优势肠道微生物群的组分迅速适应，而F。prausnitzii物种与肥胖症和糖尿病中低度炎症状态的减少直接相关，而与卡路里摄入无关。
Obesity alters gut microbiota ecology and associates with low-grade inflammation in humans. Roux-en-Y gastric bypass (RYGB) surgery is one of the most efficient procedures for the treatment of morbid obesity resulting in drastic weight loss and improvement of metabolic and inflammatory status. We analyzed the impact of RYGB on the modifications of gut microbiota and examined links with adaptations associated with this procedure. Gut microbiota was profiled from fecal samples by real-time quantitative PCR in 13 lean control subjects and in 30 obese individuals (with seven type 2 diabetics) explored before (M0), 3 months (M3), and 6 months (M6) after RYGB. Four major findings are highlighted: 1) Bacteroides/Prevotella group was lower in obese subjects than in control subjects at M0 and increased at M3. It was negatively correlated with corpulence, but the correlation depended highly on caloric intake; 2) Escherichia coli species increased at M3 and inversely correlated with fat mass and leptin levels independently of changes in food intake; 3) lactic acid bacteria including Lactobacillus/Leuconostoc/Pediococcus group and Bifidobacterium genus decreased at M3; and 4) Faecalibacterium prausnitzii species was lower in subjects with diabetes and associated negatively with inflammatory markers at M0 and throughout the follow-up after surgery independently of changes in food intake. These results suggest that components of the dominant gut microbiota rapidly adapt in a starvation-like situation induced by RYGB while the F. prausnitzii species is directly linked to the reduction in low-grade inflammation state in obesity and diabetes independently of calorie intake.
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影响因子：
7.1
作者：
Keogh, Jennifer B.;Brinkworth, Grant D.;Clifton, Peter M.
通讯作者：
Clifton, Peter M.
影响因子：
4.9
作者：
Cinti, S;De Matteis, R;Palou, A
通讯作者：
Palou, A
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
11.1
作者：
Clement, K.;Langin, D.
通讯作者：
Langin, D.
影响因子：
4.9
作者：
Korner, J.;Inabet, W.;Fabres, G.;Conwell, I. M.;McMahon, D. J.;Salas, R.;Taveras, C.;Schrope, B.;Bessler, M.
通讯作者：
Bessler, M.