Integrative Analysis of Expression Profiles of mRNA and MicroRNA Provides Insights of Cotton Response to Verticillium dahliae.
Integrative Analysis of Expression Profiles of mRNA and MicroRNA Provides Insights of Cotton Response to Verticillium dahliae.
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DOI：
10.3390/ijms23094702
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发表时间：
2022-04-24
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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棉花黄萎病是由棉花黄萎病菌（Verticilliumdahliae）引起的一种破坏性的土传维管束病害，严重影响棉花产量和品质。对棉花黄萎病菌应答基因的转录和转录后调控是植物对棉花黄萎病菌耐受性的关键。然而，与棉花抗黄萎病相关的特异性microRNAs（miRNAs）及其与靶基因的互作仍然十分有限。为研究高地黄萎病菌诱导过程中调控RNA的作用，以感病品种冀棉11号（J11）和抗病品种中棉2号（Z2）为材料，构建了棉花黄萎病菌诱导过程中调控RNA的mRNA和小RNA文库。比较转录组分析显示8330个转录本在大丽轮枝菌胁迫下差异表达，并与几个特定的生物学过程相关。此外，小RNA测序共鉴定出383个miRNA，包括330个独特的保守miRNA和53个新的miRNA。对参与响应大丽轮枝菌胁迫的调控网络的分析揭示了31个差异表达的miRNA-mRNA对，而GhmiR 395的上调和GhmiR 165的下调可能通过GhmiR 395-APS 1/3模块调节硫同化以及GhmiR 165-APS模块调节维管构型和次生细胞壁的形成而参与对大丽轮枝菌的反应。REV模块。对高地棉中mRNA和miRNA表达谱的整合分析为进一步研究棉花及其他作物抗黄萎病的调控机制奠定了基础。
Cotton Verticillium wilt, caused by the notorious fungal phytopathogen Verticillium dahliae (V. dahliae), is a destructive soil-borne vascular disease and severely decreases cotton yield and quality worldwide. Transcriptional and post-transcriptional regulation of genes responsive to V. dahliae are crucial for V. dahliae tolerance in plants. However, the specific microRNAs (miRNAs) and the miRNA/target gene crosstalk involved in cotton resistance to Verticillium wilt remain largely limited. To investigate the roles of regulatory RNAs under V. dahliae induction in upland cotton, mRNA and small RNA libraries were constructed from mocked and infected roots of two upland cotton cultivars with the V. dahliae-sensitive cultivar Jimian 11 (J11) and the V. dahliae-tolerant cultivar Zhongzhimian 2 (Z2). A comparative transcriptome analysis revealed 8330 transcripts were differentially expressed under V. dahliae stress and associated with several specific biological processes. Moreover, small RNA sequencing identified a total of 383 miRNAs, including 330 unique conserved miRNAs and 53 novel miRNAs. Analysis of the regulatory network involved in the response to V. dahliae stress revealed 31 differentially expressed miRNA–mRNA pairs, and the up-regulation of GhmiR395 and down-regulation of GhmiR165 were possibly involved in the response to V. dahliae by regulating sulfur assimilation through the GhmiR395-APS1/3 module and the establishment of the vascular pattern and secondary cell wall formation through GhmiR165-REV module, respectively. The integrative analysis of mRNA and miRNA expression profiles from upland cotton lays the foundation for further investigation of regulatory mechanisms of resistance to Verticillium wilt in cotton and other crops.
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