TFDP3 regulates the apoptosis and autophagy in breast cancer cell line MDA-MB-231.
TFDP3 regulates the apoptosis and autophagy in breast cancer cell line MDA-MB-231.
复制标题
TFDP3调节乳腺癌细胞系MDA-MB-231的凋亡和自噬
DOI：
10.1371/journal.pone.0203833
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Wang YD
中科院分区：
文献类型：
--
作者：
Ding LY;Chu M;Jiao YS;Hao Q;Xiao P;Li HH;Guo Q;Wang YD
肿瘤/睾丸抗原TFDP 3属于转录因子DP（TFDP）家族。它可以与E2 F家族分子结合形成异二聚体转录因子E2 F/TFDP复合物。该复合物是一种重要的细胞周期调节激活剂，参与调节细胞增殖、分化、凋亡等重要生理活动。此外，还发现TFDP 3是一种肿瘤相关抗原，仅在恶性肿瘤组织和正常睾丸组织中表达，与肿瘤的发生、发展密切相关。在这项研究中，我们的小组研究了TFDP 3在各种组织来源的恶性肿瘤，乳腺癌和良性乳腺病变的单核细胞样本中的表达。结果显示TFDP 3在各种组织的恶性形式中表达。此外，我们最近的研究集中在TFDP 3影响肿瘤细胞耐药性和凋亡的能力。为了进一步阐明肿瘤抵抗的机制，本研究还研究了TFDP 3的表达和肿瘤细胞自噬的调节;自噬有助于细胞科普代谢应激（如在营养不良、生长因子耗竭、缺氧或缺氧的情况下），去除错误折叠的蛋白质或有缺陷的细胞器，以防止异常蛋白质的积累;并去除细胞内病原体。我们的研究结果表明，TFDP 3的表达可以通过上调自噬关键蛋白LC 3（MAP 1 LC 3）的表达和增加恶性肿瘤化疗过程中自噬体的数量来诱导自噬。然后，由化疗药物引起的DNA和细胞器损伤被修复。因此，TFDP 3有助于肿瘤细胞抗性。当siRNA抑制TFDP 3表达时，它可以减少细胞自噬，提高肿瘤细胞对化疗药物的敏感性。
Cancer/testis antigen TFDP3 belongs to the transcription factor DP(TFDP) family. It can bind to E2F family molecules to form a heterodimeric transcription factor E2F/TFDP complex. The complex is an important regulatory activator of cell cycle, involved in the regulation of cell proliferation, differentiation, apoptosis and other important physiological activities. In addition, TFDP3 has also been found to be a tumor-associated antigen that only expresses in malignant tumor tissue and normal testicular tissue; Thus, it is closely related to tumor occurrence and development. In this study, our group investigated the expression of TFDP3 in mononuclear cell samples from a variety of tissue-derived malignant tumors, breast cancer and benign breast lesions. The results show that TFDP3 is expressed in the malignant form of various tissues. Moreover, our recent research had focused on the ability of TFDP3 to influence the drug resistance and apoptosis of tumor cells. To further clarify the mechanisms involved in tumor resistance, this study also examined the expression of TFDP3 and tumor cell autophagy regulation; Autophagy helps cells cope with metabolic stress (such as in cases of malnutrition, growth factor depletion, hypoxia or hypoxia) removes erroneously folded proteins or defective organelles to prevent the accumulation of abnormal proteins; and removes intracellular pathogens. Our results showed that TFDP3 expression can induce autophagy by up-regulating the expression of autophagic key protein LC3(MAP1LC3) and increasing the number of autophagosomes during chemotherapy of malignant tumors. Then, DNA and organelles damage caused by the chemotherapy medicine are repaired. Thus, TFDP3 contributes toward tumor cell resistance. When siRNA inhibits TFDP3 expression, it can reduce cell autophagy, improving the sensitivity of tumor cells to chemotherapy drugs.
登录
查看更多内容
影响因子：
0.3
作者：
Kasprowska-Liskiewicz, Daniela
通讯作者：
Kasprowska-Liskiewicz, Daniela
影响因子：
11.4
作者：
Romanov, Julia;Walczak, Marta;Ibiricu, Iosune;Schuechner, Stefan;Ogris, Egon;Kraft, Claudine;Martens, Sascha
通讯作者：
Martens, Sascha
TFDP3调节乳腺癌中的上皮间质转变。
影响因子：
3.7
作者：
Yin K;Liu Y;Chu M;Wang Y
通讯作者：
Wang Y
DOI：
10.1093/annonc/mdt303
发表时间：
2013-09
期刊：
Annals of oncology : official journal of the European Society for Medical Oncology
影响因子：
--
作者：
Goldhirsch A;Winer EP;Coates AS;Gelber RD;Piccart-Gebhart M;Thürlimann B;Senn HJ;Panel members
通讯作者：
Panel members
影响因子：
12.4
作者：
Ingram, L.;Munro, S.;La Thangue, N. B.
通讯作者：
La Thangue, N. B.