Rothia from the Human Nose Inhibit Moraxella catarrhalis Colonization with a Secreted Peptidoglycan Endopeptidase.
Rothia from the Human Nose Inhibit Moraxella catarrhalis Colonization with a Secreted Peptidoglycan Endopeptidase.
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DOI：
10.1128/mbio.00464-23
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发表时间：
2023-04-25
期刊：
影响因子：
6.4
通讯作者：
Currie, Cameron R.
中科院分区：
文献类型：
--
作者：
Stubbendieck, Reed M.;Dissanayake, Eishika;Burnham, Peter M.;Zelasko, Susan E.;Temkin, Mia I.;Wisdorf, Sydney S.;Vrtis, Rose F.;Gern, James E.;Currie, Cameron R.
关键词：
卡他莫拉氏菌几乎只在人类呼吸道内发现。这种疾病与耳朵感染和呼吸道疾病的发展有关，包括过敏和哮喘。鉴于卡他分支杆菌的生态分布有限，我们假设我们可以利用没有卡他分支杆菌的健康儿童的鼻部微生物群来识别可能代表潜在治疗来源的细菌。与有感冒症状的儿童和卡他支原体相比，健康儿童鼻腔中的罗瑟氏菌含量更高。我们从鼻腔标本中培养出Rothia Dentocariosa和“Rothia sisimililaginosa”，大多数菌株在体外都能完全抑制卡他分支杆菌的生长，而Rothia aeria的菌株对卡他支原体的抑制能力有所不同。利用比较基因组学和蛋白质组学方法，我们鉴定了一种可能的被称为分泌型抗原A(SAGA)的肽聚糖水解酶。该蛋白在齿状根结线虫和相似粘性根结线虫的分泌蛋白质组中的相对丰度高于非抑制的斑点落叶松毛虫的相对丰度，提示该蛋白可能参与了卡他毛虫的抑制作用。我们在大肠杆菌中从相似粘菌中产生了SAGA，并证实了其降解卡他林多糖肽聚糖和抑制其生长的能力。然后，我们在呼吸道上皮的气液界面培养模型中证明了Aeria和R.sisimililaginosa降低了卡他毛支原体的水平。综上所述，我们的结果表明，在活体内，Rothia限制了卡他毛杆菌在人体呼吸道的定植。
Moraxella catarrhalis is found almost exclusively within the human respiratory tract. This pathobiont is associated with ear infections and the development of respiratory illnesses, including allergies and asthma. Given the limited ecological distribution of M. catarrhalis, we hypothesized that we could leverage the nasal microbiomes of healthy children without M. catarrhalis to identify bacteria that may represent potential sources of therapeutics. Rothia was more abundant in the noses of healthy children compared to children with cold symptoms and M. catarrhalis. We cultured Rothia from nasal samples and determined that most isolates of Rothia dentocariosa and “Rothia similmucilaginosa” were able to fully inhibit the growth of M. catarrhalis in vitro, whereas isolates of Rothia aeria varied in their ability to inhibit M. catarrhalis. Using comparative genomics and proteomics, we identified a putative peptidoglycan hydrolase called secreted antigen A (SagA). This protein was present at higher relative abundance in the secreted proteomes of R. dentocariosa and R. similmucilaginosa than in those from non-inhibitory R. aeria, suggesting that it may be involved in M. catarrhalis inhibition. We produced SagA from R. similmucilaginosa in Escherichia coli and confirmed its ability to degrade M. catarrhalis peptidoglycan and inhibit its growth. We then demonstrated that R. aeria and R. similmucilaginosa reduced M. catarrhalis levels in an air-liquid interface culture model of the respiratory epithelium. Together, our results suggest that Rothia restricts M. catarrhalis colonization of the human respiratory tract in vivo.
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影响因子：
5.4
作者：
Berrington, William R.;Iyer, Ravi;Wells, Richard D.;Smith, Kelly D.;Skerrett, Shawn J.;Hawn, Thomas R.
通讯作者：
Hawn, Thomas R.
DOI：
10.1007/978-1-4419-7185-2_9
发表时间：
2011-01-01
期刊：
HOT TOPICS IN INFECTION AND IMMUNITY IN CHILDREN VII
影响因子：
--
作者：
Aebi, Christoph
通讯作者：
Aebi, Christoph
鼻病毒增加卡他莫拉菌对呼吸道上皮的粘附。
影响因子：
5.7
作者：
通讯作者：
--
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
5.4
作者：
Bomar L;Brugger SD;Lemon KP
通讯作者：
Lemon KP