Simultaneous improvement for four quality traits of Zhenshan 97, an elite parent of hybrid rice, by molecular marker-assisted selection
Simultaneous improvement for four quality traits of Zhenshan 97, an elite parent of hybrid rice, by molecular marker-assisted selection
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杂交水稻优良亲本珍山97 4个品质性状的分子标记辅助选择同步改良
DOI：
10.1007/s00122-002-1023-0
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Zhang Qifa
中科院分区：
文献类型：
--
作者：
Zhou Penghui;Tan Yifang;He Yuqing;Xu Caiguo;Zhang Qifa
“震山97”是我国多个广泛应用的水稻杂交种的母本。然而，该品系由于直链淀粉含量（AC）高、凝胶稠度（GC）硬、糊化温度（GT）低以及白垩质胚乳而质量较差。已经确定烹饪和食用质量的三个性状AC、GC和GT是由蜡座和/或紧密连锁的基因组区域控制的。在这项研究中，我们通过从具有中等AC、软GC和高GT的恢复系明慧63（wx-MH）中引入Waxy基因区域来改善镇山97的食味和烹饪品质。 wx-MH片段通过3次回交和1次自交转移到镇山97B，然后通过杂交和1次回交从镇山97B转移到镇山97A。该系列应用分子标记辅助选择来选择携带wx-MH的个体，以鉴定Waxy和侧翼标记之间的重组，并恢复轮回亲本的遗传背景。根据标记基因型，镇山97B和镇山97A的改良版，或镇山97B(wx-MH)和镇山97A(wx-MH)，除了蜡质区长度小于6.1 cM外，与原版相同。所选品系及其与明徽63或汕优63(wx-MH)的杂交种表现出AC降低、GC和GT增加以及籽粒不透明度降低。农艺性能田间检测表明，镇山97B（wx-MH）和汕优63（wx-MH）除粒重显着下降外，与原品种基本相同。 AC、GA、GT和不透明度的同时提高表明蜡质区对4个品质性状影响较大。镇山 97 A 和 B 的改良版本应该可以立即用于杂交水稻生产。
"Zhenshan 97" is the female parent of a number of widely used hybrids for rice production in China. However, this line is of poor quality because of a high amylose content (AC), a hard gel consistency (GC) and a low gelatinization temperature (GT), together with a chalky endosperm. It had been determined that the three traits for cooking and eating quality, AC, GC and GT, are controlled by theWaxylocus and/or the tightly linked genomic region. In this study we improved the eating and cooking quality of Zhenshan 97 by introgressing theWaxygene region from Minghui 63 (wx-MH), a restorer line, that has medium AC, soft GC and high GT. The wx-MH fragment was transferred to Zhenshan 97B by three backcrosses and one selfing, then from Zhenshan 97B to Zhenshan 97A by a cross and a backcross. Molecular marker-assisted selection was applied in the series to select for individuals carrying wx-MH, to identify recombination between theWaxyand flanking markers, and also to recover the genetic background of the recurrent parent. According to the marker genotypes, the improved versions of Zhenshan 97B and Zhenshan 97A, or Zhenshan 97B(wx-MH) and Zhenshan 97A(wx-MH), were the same as the originals except for theWaxyregion of less than 6.1 cM in length. The selected lines and their hybrids with Minghui 63, or Shanyou 63(wx-MH), showed a reduced AC and an increased GC and GT, coupled with a reduced grain opacity. Field examinations of agronomic performance revealed that Zhenshan 97B(wx-MH) and Shanyou 63(wx-MH) were essentially the same as the originals except for a significant decrease in grain weight. The simultaneous improvement of AC, GA, GT and opacity, indicated that theWaxyregion had major effects on the four quality traits. The improved versions of Zhenshan 97 A and B should be immediately useful in hybrid rice production.
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影响因子：
5.4
作者：
Tan, YF;Xing, YZ;Zhang, QF
通讯作者：
Zhang, QF
影响因子：
5.4
作者：
Qifa Zhang;Y. J. Gao;S. Yang;R. Ragab;M. Maroof;Zhaohong Li
通讯作者：
Qifa Zhang;Y. J. Gao;S. Yang;R. Ragab;M. Maroof;Zhaohong Li
DOI：
10.1007/bf00280220
发表时间：
1993-10-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
WU, KS;TANKSLEY, SD
通讯作者：
TANKSLEY, SD
影响因子：
5.4
作者：
Tan, YF;Li, JX;Zhang, Q
通讯作者：
Zhang, Q
影响因子：
14.9
作者：
VOS, P;HOGERS, R;ZABEAU, M
通讯作者：
ZABEAU, M