Intracellular and Extracellular Recording of Spontaneous Action Potentials in Mammalian Neurons and Cardiac Cells with 3D Plasmonic Nanoelectrodes.
Intracellular and Extracellular Recording of Spontaneous Action Potentials in Mammalian Neurons and Cardiac Cells with 3D Plasmonic Nanoelectrodes.
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DOI：
10.1021/acs.nanolett.7b01523
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发表时间：
2017-06-14
期刊：
影响因子：
10.8
通讯作者：
De Angelis F
中科院分区：
文献类型：
--
作者：
Dipalo M;Amin H;Lovato L;Moia F;Caprettini V;Messina GC;Tantussi F;Berdondini L;De Angelis F
三维垂直微米和纳米结构可以增强多电极阵列的信号质量，并有望成为研究大型生电细胞网络的主要方法。到目前为止，进入细胞内环境的途径是通过自发穿孔、电穿孔或微/纳米结构的表面功能化。然而，这些方法由于其内在特征仍然受到一些限制，限制了它们的广泛使用。在这里，我们展示了通过垂直纳米电极与等离子体光孔相结合，连续记录自发活动的哺乳动物神经元和 HL-1 心脏来源细胞的每个电极位点的细胞外和类细胞内动作电位的能力。我们展示了具有非常好的信噪比的长期稳定的录音。此外，等离子体光穿孔不会干扰自发电活动。它甚至可以在穿孔过程中进行连续记录，并且可以通过部分细胞穿孔来调节细胞外和细胞内的贡献。
Three-dimensional vertical micro- and nanostructures can enhance the signal quality of multielectrode arrays and promise to become the prime methodology for the investigation of large networks of electrogenic cells. So far, access to the intracellular environment has been obtained via spontaneous poration, electroporation, or by surface functionalization of the micro/nanostructures; however, these methods still suffer from some limitations due to their intrinsic characteristics that limit their widespread use. Here, we demonstrate the ability to continuously record both extracellular and intracellular-like action potentials at each electrode site in spontaneously active mammalian neurons and HL-1 cardiac-derived cells via the combination of vertical nanoelectrodes with plasmonic optoporation. We demonstrate long-term and stable recordings with a very good signal-to-noise ratio. Additionally, plasmonic optoporation does not perturb the spontaneous electrical activity; it permits continuous recording even during the poration process and can regulate extracellular and intracellular contributions by means of partial cellular poration.
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影响因子：
10.2
作者：
Kuo CW;Chueh DY;Chen P
通讯作者：
Chen P
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
4.6
作者：
Rabieh N;Ojovan SM;Shmoel N;Erez H;Maydan E;Spira ME
通讯作者：
Spira ME
影响因子：
3.3
作者：
Lampl, I;Yarom, Y
通讯作者：
Yarom, Y
影响因子：
4.6
作者：
Kampasi K;Stark E;Seymour J;Na K;Winful HG;Buzsáki G;Wise KD;Yoon E
通讯作者：
Yoon E