Graphene FET Sensors for Alzheimer's Disease Protein Biomarker Clusterin Detection.
Graphene FET Sensors for Alzheimer's Disease Protein Biomarker Clusterin Detection.
复制标题
DOI：
10.3389/fmolb.2021.651232
复制
发表时间：
2021
影响因子：
5
通讯作者：
Awan SA
中科院分区：
文献类型：
--
作者：
Bungon T;Haslam C;Damiati S;O'Driscoll B;Whitley T;Davey P;Siligardi G;Charmet J;Awan SA
我们报告了石墨烯场效应晶体管（GFET）生物传感器的制造和表征，用于检测阿尔茨海默病（AD）的一种突出的蛋白质生物标志物-阿糖胞苷。GFET传感器制作在Si/SiO2衬底上，使用光刻图案化和金属剥离技术与蒸发的铬和溅射的金接触。在装置上进行拉曼光谱以确定石墨烯的质量。退火GFET以改善其性能，然后通过用接头分子和抗-磷脂抗体固定石墨烯表面来官能化通道。还通过使用Diamond B23光束线的高度准直微束光的吸收光谱法独立地验证了连接剂分子的浓度。检测是通过抗体与1至100 pg/mL不同浓度的促性腺激素抗原之间的结合反应以及使用人绒毛膜促性腺激素（hCG）（某些癌症的糖蛋白风险生物标志物）的特异性测试来实现的。使用直流（DC）4-探针电阻（4-PER）测量表征GFET，其证明生物传感器的检测限为约300 fg/mL（4 fM）。与背门控狄拉克电压偏移与不同浓度的pneumaterin的比较显示4-PER测量更准确，目前，并指出进一步优化我们的下一代GFET传感器的制造工艺的要求。因此，我们已经成功地制作了一套有前途的GFET生物传感器用于检测蛋白质生物标志物。开发的GFET生物传感器是完全通用的，也有可能被应用于各种其他疾病的检测应用，如帕金森氏症，癌症和心血管。
We report on the fabrication and characterisation of graphene field-effect transistor (GFET) biosensors for the detection of Clusterin, a prominent protein biomarker of Alzheimer’s disease (AD). The GFET sensors were fabricated on Si/SiO2 substrate using photolithographic patterning and metal lift-off techniques with evaporated chromium and sputtered gold contacts. Raman Spectroscopy was performed on the devices to determine the quality of the graphene. The GFETs were annealed to improve their performance before the channels were functionalized by immobilising the graphene surface with linker molecules and anti-Clusterin antibodies. Concentration of linker molecules was also independently verified by absorption spectroscopy using the highly collimated micro-beam light of Diamond B23 beamline. The detection was achieved through the binding reaction between the antibody and varying concentrations of Clusterin antigen from 1 to 100 pg/mL, as well as specificity tests using human chorionic gonadotropin (hCG), a glycoprotein risk biomarker of certain cancers. The GFETs were characterized using direct current (DC) 4-probe electrical resistance (4-PER) measurements, which demonstrated a limit of detection of the biosensors to be ∼ 300 fg/mL (4 fM). Comparison with back-gated Dirac voltage shifts with varying concentration of Clusterin show 4-PER measurements to be more accurate, at present, and point to a requirement for further optimisation of the fabrication processes for our next generation of GFET sensors. Thus, we have successfully fabricated a promising set of GFET biosensors for the detection of Clusterin protein biomarker. The developed GFET biosensors are entirely generic and also have the potential to be applied to a variety of other disease detection applications such as Parkinson’s, cancer, and cardiovascular.
登录
查看更多内容
DOI：
10.1016/s0006-291x(84)80190-4
发表时间：
1984-01-01
影响因子：
3.1
作者：
GLENNER, GG;WONG, CW
通讯作者：
WONG, CW
影响因子：
10.8
作者：
Blake, Peter;Brimicombe, Paul D.;Novoselov, Kostya S.
通讯作者：
Novoselov, Kostya S.
影响因子：
56.9
作者：
Bunch, J. Scott;van der Zande, Arend M.;McEuen, Paul L.
通讯作者：
McEuen, Paul L.
影响因子：
10.8
作者：
Balandin, Alexander A.;Ghosh, Suchismita;Lau, Chun Ning
通讯作者：
Lau, Chun Ning
影响因子：
2.1
作者：
Bolotin, K. I.;Sikes, K. J.;Stormer, H. L.
通讯作者：
Stormer, H. L.