Pre- and postnatal developmental changes in hypothalamic content of rat growth hormone-releasing factor.
Pre- and postnatal developmental changes in hypothalamic content of rat growth hormone-releasing factor.
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大鼠生长激素释放因子下丘脑含量的产前和产后发育变化。
DOI：
10.1210/endo-120-2-525
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发表时间：
1987
期刊：
影响因子：
4.8
通讯作者：
L. Frohman
中科院分区：
文献类型：
--
作者：
J. Jansson;K. Ishikawa;H. Katakami;L. Frohman
采用特异性大鼠下丘脑gh释放因子（GRF）免疫组化法，观察了大鼠出生前后下丘脑gh释放因子（GRF）的发生情况。妊娠第17天GRF含量未检测到（低于10 pg/下丘脑），妊娠第18-20天GRF含量增加至30-65 pg/下丘脑。出生后20 ~ 50天下丘脑GRF含量比0 ~ 20天和50 ~ 90天增加得更快。50 ~ 90日龄大鼠下丘脑GRF含量为900 ~ 1300 pg/，产后无性别差异。RIA测量的下丘脑生长抑素水平显示出与大鼠GRF相似的发育模式。胎儿和成年大鼠下丘脑提取物的GRF免疫反应性与合成大鼠GRF的HPLC保留时间相同。抗大鼠GRF血清可显著降低胎鼠妊娠第21天和新生幼鼠出生后4小时的血浆GH水平。GRF被动免疫在出生后6-9小时和出生后第3天对血浆GH的抑制更为明显（75-85%）。结果表明，免疫反应性GRF在胎儿和新生大鼠的下丘脑中存在可测量的水平，在化学上与合成大鼠GRF没有区别，并且早在胎儿生命的第21天就表现出生物学效应。
The ontogenesis of hypothalamic GH-releasing factor (GRF) in pre- and postnatal rats was examined by means of a specific rat GRF RIA. Whereas GRF content was undetectable (less than 10 pg/hypothalamus) on day 17 of gestation, it increased to 30-65 pg/hypothalamus during days 18-20. During postnatal life, hypothalamic GRF content increased more rapidly during days 20-50 than during days 0-20 or 50-90. GRF content was 900-1300 pg/hypothalamus in 50- to 90-day-old rats, and there was no consistent sex difference during postnatal life. Hypothalamic somatostatin levels, as measured by RIA, showed a developmental pattern similar to that of rat GRF. GRF immunoreactivity in hypothalamic extracts from fetal as well as adult rats exhibited HPLC retention times identical to that of synthetic rat GRF. Administration of antirat GRF serum produced a significant decrease in plasma GH levels in fetal rats on day 21 of gestation and in newborn pups 4 h after birth. Passive immunization against GRF caused a more marked suppression of plasma GH (75-85%) 6-9 h after birth and on postnatal day 3. The results demonstrate that immunoreactive GRF is present in measurable levels in the hypothalami of fetal and newborn rats, is chemically indistinguishable from synthetic rat GRF, and exhibits biological effects as early as day 21 of fetal life.
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甲状腺功能减退症时下丘脑生长激素释放激素含量和垂体反应性降低。
DOI：
10.1172/jci112490
发表时间：
1986
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Katakami,H;Downs,TR;Frohman,LA
通讯作者：
Frohman,LA
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影响因子：
3.6
作者：
Wehrenberg,WB;Baird,A;Ying,SY;Ling,N
通讯作者：
Ling,N
影响因子：
56.9
作者：
GUILLEMIN, R;BRAZEAU, P;WEHRENBERG, WB
通讯作者：
WEHRENBERG, WB
影响因子：
4.8
作者：
Khorram,O;DePalatis,LR;McCann,SM
通讯作者：
McCann,SM
大鼠下丘脑和垂体α-黑素细胞刺激激素和生长抑素含量的产前和产后发育变化。
DOI：
10.1159/000242037
发表时间：
1984
期刊：
Biology of the neonate
影响因子：
--
作者：
Khorram,O;McCann,SM
通讯作者：
McCann,SM