Clathrin-mediated endocytosis facilitates the internalization of Magnaporthe oryzae effectors into rice cells.
Clathrin-mediated endocytosis facilitates the internalization of Magnaporthe oryzae effectors into rice cells.
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DOI：
10.1093/plcell/koad094
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发表时间：
2023-06-26
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影响因子：
--
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中科院分区：
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--
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真菌和卵菌将效应子传递到活的植物细胞中，以抑制防御并控制感染所需的植物过程。关于这些病原体将效应蛋白跨质膜转运到植物细胞质中的机制知之甚少。稻瘟病菌在易位之前将细胞质效应物分泌到专门的生物营养界面复合物（BIC）中。在这里，我们表明，BIC内的细胞质效应包装成点状膜效应隔室，偶尔在宿主细胞质中观察到。用荧光标记的蛋白质在水稻（Oryza sativa）中进行的活细胞成像显示，这些效应点与植物质膜和网格蛋白轻链1（网格蛋白介导的内吞作用（CME）的一个组分）共定位。使用病毒诱导的基因沉默和化学处理抑制CME导致在肿胀的BIC中缺乏效应子点的细胞质效应子。相比之下，荧光标记共定位，基因沉默，和化学抑制剂的研究未能支持一个主要的作用，网格蛋白独立的内吞效应易位。效应子定位模式表明，细胞质效应子易位发生在附着胞下方的侵入性菌丝生长之前。总之，这项研究提供了证据，细胞质效应易位是由CME介导的BIC和M。植物内吞作用的抑制因子。活细胞成像，病毒诱导的基因沉默，和药理学研究提供的证据表明，网格蛋白介导的内吞作用促进稻瘟病菌效应器内水稻细胞的易位。
Fungi and oomycetes deliver effectors into living plant cells to suppress defenses and control plant processes needed for infection. Little is known about the mechanism by which these pathogens translocate effector proteins across the plasma membrane into the plant cytoplasm. The blast fungus Magnaporthe oryzae secretes cytoplasmic effectors into a specialized biotrophic interfacial complex (BIC) before translocation. Here, we show that cytoplasmic effectors within BICs are packaged into punctate membranous effector compartments that are occasionally observed in the host cytoplasm. Live cell imaging with fluorescently labeled proteins in rice (Oryza sativa) showed that these effector puncta colocalize with the plant plasma membrane and with CLATHRIN LIGHT CHAIN 1, a component of clathrin-mediated endocytosis (CME). Inhibiting CME using virus-induced gene silencing and chemical treatments resulted in cytoplasmic effectors in swollen BICs lacking effector puncta. By contrast, fluorescent marker colocalization, gene silencing, and chemical inhibitor studies failed to support a major role for clathrin-independent endocytosis in effector translocation. Effector localization patterns indicated that cytoplasmic effector translocation occurs underneath appressoria before invasive hyphal growth. Taken together, this study provides evidence that cytoplasmic effector translocation is mediated by CME in BICs and suggests a role for M. oryzae effectors in coopting plant endocytosis. Live cell imaging, virus-induced gene silencing, and pharmacological studies provide evidence that clathrin-mediated endocytosis facilitates translocation of Magnaporthe effectors inside rice cells.
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影响因子：
7.7
作者：
Dagdas YF;Pandey P;Tumtas Y;Sanguankiattichai N;Belhaj K;Duggan C;Leary AY;Segretin ME;Contreras MP;Savage Z;Khandare VS;Kamoun S;Bozkurt TO
通讯作者：
Bozkurt TO
影响因子：
6.4
作者：
Gladieux P;Condon B;Ravel S;Soanes D;Maciel JLN;Nhani A Jr;Chen L;Terauchi R;Lebrun MH;Tharreau D;Mitchell T;Pedley KF;Valent B;Talbot NJ;Farman M;Fournier E
通讯作者：
Fournier E
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
2
作者：
Bolte, S;Talbot, C;Satiat-Jeunemaitre, B
通讯作者：
Satiat-Jeunemaitre, B
影响因子：
9.4
作者：
Collemare, Jerome;Pianfetti, Mikael;Boehnert, Heidi U.
通讯作者：
Boehnert, Heidi U.