Alternative Splicing Dynamics of the Hypothalamus-Pituitary-Ovary Axis During Pubertal Transition in Gilts.
Alternative Splicing Dynamics of the Hypothalamus-Pituitary-Ovary Axis During Pubertal Transition in Gilts.
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后备母猪青春期过渡期间下丘脑-垂体-卵巢轴的选择性剪接动态
DOI：
10.3389/fgene.2021.592669
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发表时间：
2021
影响因子：
3.7
通讯作者：
Yuan X
中科院分区：
文献类型：
--
作者：
Pan X;Li Q;Chen D;Gong W;Li N;Jiang Y;Zhang H;Chen Y;Yuan X
哺乳动物青春期的时间标志着繁殖成为可能的时间点。青春期发育的时机不当可能会对随后的健康结果产生一系列负面影响。选择性剪接（AS）不仅是基因转录的一个重要因素，而且据报道它在青春期的时间安排中也起着重要作用。然而，到目前为止，在青春期开始AS的变化和动力学是非常少探索。在本研究中，我们使用后备母猪作为研究模型，调查AS和差异表达AS（DEAS）事件在下丘脑-垂体-卵巢（HPO）轴跨青春期前，青春期中和青春期后的动态。我们在青春期前、青春期内和青春期后的下丘脑、垂体和卵巢中分别检测到3,390、6,098和9,085例DEAS事件。在整个HPO轴中，我们还分别在青春期前、青春期内和青春期后阶段确定了22，889、22，857和21，055起DEAS事件。进一步的分析表明，差异剪接基因（DSG）与分期DEAS事件可能是丰富的催产素信号通路，甲状腺激素信号通路，GnRH信号通路，卵母细胞减数分裂信号通路。在整个HPO轴上与DEAS事件相关的DSG富含内吞信号通路、MAPK信号通路和Rap 1信号通路。而且。TAC 1、TACR 3、CYP 19 A1、ESR 1、ESRRA和FSHR的AS可能在青春期开始时调节某些HPO组织的功能。总的来说，AS动力学和DEAS事件在下丘脑，垂体和卵巢中的全面分析，在青春期前，青春期内和青春期后阶段的猪。这些发现可能会增强我们对AS如何调节青春期的认识，并为哺乳动物青春期时间的分子机制提供新的线索。
The timing of puberty in mammals marks the point at which reproduction becomes possible. Abnormalities in the timing of puberty may exert a series of negative effects on subsequent health outcomes. Alternative splicing (AS) has not only emerged as a significant factor in the transcription of genes but it is also reported to play a role in the timing of puberty. However, to date, the changes and dynamics of AS during the onset of puberty is extremely seldom explored. In the present study, we used gilts as a research model to investigated the dynamics of AS and differentially expressed AS (DEAS) events within the hypothalamus–pituitary–ovary (HPO) axis across pre-, in-, and post-puberty. We detected 3,390, 6,098, and 9,085 DEAS events in the hypothalamus, pituitary, and ovary when compared across pre-, in-, and post-pubertal stages, respectively. Within the entire HPO axis, we also identified 22,889, 22,857, and 21,055 DEAS events in the pre-, in-, and post-pubertal stages, respectively. Further analysis revealed that the differentially spliced genes (DSGs) associated with staged DEAS events were likely to be enriched in the oxytocin signaling pathway, thyroid hormone signaling pathway, GnRH signaling pathway, and oocyte meiosis signaling pathway. The DSGs associated with DEAS events across the entire HPO axis were enriched in endocytosis signaling pathway, the MAPK signaling pathway, and the Rap1 signaling pathway. Moreover. the ASs of TAC1, TACR3, CYP19A1, ESR1, ESRRA, and FSHR were likely to regulate the functions of the certain HPO tissues during the onset of puberty. Collectively, the AS dynamics and DEAS events were comprehensively profiled in hypothalamus, pituitary, and ovary across the pre-, in-, and post-pubertal stages in pigs. These findings may enhance our knowledge of how puberty is regulated by AS and shed new light on the molecular mechanisms underlying the timing of puberty in mammals.
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影响因子：
4.6
作者：
Gioacchini, Giorgia;Marisaldi, Luca;Carnevali, Oliana
通讯作者：
Carnevali, Oliana
影响因子：
4.1
作者：
Balasubramanian, Ravikumar;Dwyer, Andrew;Crowley, William F., Jr.
通讯作者：
Crowley, William F., Jr.
影响因子：
13.3
作者：
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通讯作者：
Tena-Sempere, M.
影响因子：
8
作者：
Euling, Susan Y.;Herman-Giddens, Marcia E.;Swan, Shanna H.
通讯作者：
Swan, Shanna H.
影响因子：
4.2
作者：
Fan, Yali;Li, Jianying;Wang, Juanhong
通讯作者：
Wang, Juanhong