Comparison of fluoride modified zirconia with ceramic hydroxyapatite for preparative scale purification of immunoglobulin from serum albumin.
Comparison of fluoride modified zirconia with ceramic hydroxyapatite for preparative scale purification of immunoglobulin from serum albumin.
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氟化物改性氧化锆与陶瓷羟基磷灰石用于从血清白蛋白中制备规模纯化免疫球蛋白的比较。
DOI：
10.1080/10826069808010123
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Flickinger,MC
中科院分区：
文献类型：
--
作者：
Mullick,A;Flickinger,MC
研究了50 μm氟改性氧化锆颗粒作为一种易于清洗和蒸汽灭菌的低压制备级免疫球蛋白纯化固定相的实用性，并将其性能与40 μm陶瓷羟基磷灰石II型（cHAp II）颗粒进行了比较。平衡批次的牛血清白蛋白的支持物的结合能力单调下降，增加吸附pH值，而牛IgG是独立的pH值，在研究的pH范围内。通过穿透分析测定的动态结合容量对于氟化物改性的氧化锆为29 mg BSA/g氧化锆，对于cHAp II为8.6 mg BSA/g cHAp II。研究了线性梯度条件对氟改性氧化锆分离牛血清白蛋白和IgG的影响。同样的分离可以在更短的时间内完成，并具有更好的分辨率cHAp II。一组模型蛋白质的保留数据的分析表明，即使两个支持保留蛋白质的混合模式相互作用（阳离子，阴离子和配体交换），氟化物改性氧化锆的保留行为是不类似的cHAp II。结果还表明，蛋白质吸附-脱附动力学上的氟化物改性的氧化锆是速率限制和控制的分散的蛋白质洗脱曲线。用0.25 M NaOH清洗和蒸汽灭菌两种支持物时，对牛血清白蛋白的批次结合能力无明显影响。用0.25 M NaOH（总计700-1000柱体积）原位常规清洁氧化锆颗粒，对色谱完整性无任何可观察到的影响。
The utility of 50 μm fluoride modified zirconia particles as an easily cleaned and steam sterilizable low pressure preparative scale stationary phase for immunoglobulin purification was investigated and its performance was compared with 40 μm ceramic hydroxyapatite type II (cHAp II) particles. The equilibrium batch binding capacity of both supports for bovine serum albumin decreases monotonically with increase in the adsorption pH, while that for bovine IgG is independent of pH, in the pH ranges studied. The dynamic binding capacity, as determined by breakthrough analysis, was 29 mg BSA/g zirconia for fluoride modified zirconia and 8.6 mg BSA/g cHAp II for cHAp II. Linear gradient conditions were developed for the separation of BSA and IgG on fluoride modified zirconia. The same separation could be accomplished in a shorter time and with better resolution on cHAp II.The analysis of retention data for a set of model proteins suggests that, even though both supports retain proteins by mixed mode interactions (cation, anion and ligand exchange), the retention behavior of fluoride modified zirconia is not analogous to that of cHAp II. The results also indicate that protein adsorption-desorption kinetics on fluoride modified zirconia are rate limiting and control the dispersion of the protein elution profile. There was no observable effect on the batch binding capacity for bovine serum albumin on cleaning with 0.25 M NaOH and steam sterilization for the two supports. Zirconia particles were routinely cleaned,in situ, with 0.25 M NaOH (a total of 700–1000 column volumes) without any observable effect on the chromatographic integrity.
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影响因子：
--
作者：
Bukovsky,J;Kennett,RH
通讯作者：
Kennett,RH
通过高性能羟基磷灰石色谱法分离 IgG 独特型。
影响因子：
--
作者：
H. Juarez;G. S. Ott;J. C. Chen;T. L. Brooks;L. Stanker
通讯作者：
L. Stanker
类淋巴母细胞产生的免疫球蛋白：通过羟基磷灰石层析从培养基中制备纯化。
影响因子：
2.9
作者：
Gary J. Smith;Richard D. McFarland;Howard M. Reisner;Gordon S. Hudson
通讯作者：
Gordon S. Hudson
影响因子：
2.9
作者：
GORBUNOFF, MJ;TIMASHEFF, SN
通讯作者：
TIMASHEFF, SN
影响因子：
2.9
作者：
GORBUNOFF, MJ
通讯作者：
GORBUNOFF, MJ