Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac.
Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac.
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分枝杆菌的鉴定和测序。
DOI：
10.1371/journal.pone.0185721
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Luo F
中科院分区：
文献类型：
--
作者：
Li Y;Chen W;Wang Y;Luo K;Li Y;Bai L;Luo F
二氯喹啉酸是一种广泛应用于水稻田的除草剂。不幸的是，二氯喹啉酸残留物对许多作物/蔬菜具有植物毒性。二氯喹啉酸在自然界中的降解非常缓慢。另一方面，利用细菌降解二氯喹啉酸是减少其污染的有效方法。本研究从二氯喹啉酸污染土壤中分离到一株二氯喹啉酸生物修复细菌F4。通过形态特征和16 S rRNA基因序列分析，确定F4为分枝杆菌属（Mycobacterium sp.）。研究了温度、pH、接种量和二氯喹啉酸初始浓度对F4生长和降解效率的影响，确定了F4降解二氯喹啉酸的最佳条件。在最佳降解条件下，F4对初始浓度为50 mg/L的二氯喹啉酸在7 d内降解率达97.38%.室内盆栽试验表明，降解产物对烟草无药害。通过对F4降解二氯喹啉酸产物的分析，提出F4降解二氯喹啉酸的途径有两种：一种是甲基化途径，另一种是脱氯途径。此外，我们还通过单分子测序和从头组装的方法重建了F4的全基因组。我们在F4基因组中鉴定了77种甲基转移酶和8种脱卤酶，以支持我们假设的降解途径。
Quinclorac is a widely used herbicide in rice filed. Unfortunately, quinclorac residues are phytotoxic to many crops/vegetables. The degradation of quinclorac in nature is very slow. On the other hand, degradation of quinclorac using bacteria can be an effective and efficient method to reduce its contamination. In this study, we isolated a quinclorac bioremediation bacterium strain F4 from quinclorac contaminated soils. Based on morphological characteristics and 16S rRNA gene sequence analysis, we identified strain F4 as Mycobacterium sp. We investigated the effects of temperature, pH, inoculation size and initial quinclorac concentration on growth and degrading efficiency of F4 and determined the optimal quinclorac degrading condition of F4. Under optimal degrading conditions, F4 degraded 97.38% of quinclorac from an initial concentration of 50 mg/L in seven days. Our indoor pot experiment demonstrated that the degradation products were non-phytotoxic to tobacco. After analyzing the quinclorac degradation products of F4, we proposed that F4 could employ two pathways to degrade quinclorac: one is through methylation, the other is through dechlorination. Furthermore, we reconstructed the whole genome of F4 through single molecular sequencing and de novo assembly. We identified 77 methyltransferases and eight dehalogenases in the F4 genome to support our hypothesized degradation path.
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影响因子：
2.5
作者：
Grossmann, K
通讯作者：
Grossmann, K
DOI：
10.1080/03601230600805873
发表时间：
2006-01-01
影响因子：
2
作者：
Lu, Zhenmei;Min, Hang;Ye, Yangfang
通讯作者：
Ye, Yangfang
能够降解二氯喹啉酸和苯磺隆除草剂混合物的菌株的表征
影响因子：
5.7
作者：
Lue Zhen-Mei;Li Zi-Mu;Min Hang
通讯作者：
Min Hang
影响因子：
8.8
作者：
Pareja, Lucia;Perez-Parada, Andres;Fernandez-Alba, Amadeo R.
通讯作者：
Fernandez-Alba, Amadeo R.
影响因子：
8.8
作者：
Pinna, Maria Vittoria;Pusino, Alba
通讯作者：
Pusino, Alba