Suboptimal evolutionary novel environments promote singular altered gravity responses of transcriptome during Drosophila metamorphosis.
Suboptimal evolutionary novel environments promote singular altered gravity responses of transcriptome during Drosophila metamorphosis.
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DOI：
10.1186/1471-2148-13-133
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发表时间：
2013-06-27
影响因子：
3.4
通讯作者：
Medina FJ
中科院分区：
文献类型：
--
作者：
Herranz R;Larkin OJ;Hill RJ;Lopez-Vidriero I;van Loon JJ;Medina FJ
先前的实验表明，国际空间站（ISS）上的重力降低会导致果蝇基因表达的重要改变。这些变化被证明与空间飞行相关的环境制约因素密切相关。在这里，我们使用了一系列不同的技术进行地面模拟微重力效应，以评估次优环境条件对果蝇基因表达的影响，在减少重力。使用“基因表达动力学检查器”（GEDI）自组织图进行的全球综合分析显示，在使用不同环境条件或微重力/超重力模拟装置时，转录组的反应程度不同。虽然在每种模拟技术中受影响的基因是不同的，但我们发现，在每种情况下，相同的基因本体组，包括至少一个与行为，应激反应或器官发生相关的大型多基因家族，都是过度代表的。这些结果表明，转录组作为一个整体可以微调重力。在最佳环境条件下，重力的改变对基因表达只有轻微的影响，但当环境条件远非最佳时，基因表达必须大大调整，影响变得更加强大，这可能与生物体缺乏暴露于进化新环境（如无重力环境）的经验有关。
Previous experiments have shown that the reduced gravity aboard the International Space Station (ISS) causes important alterations in Drosophila gene expression. These changes were shown to be intimately linked to environmental space-flight related constraints. Here, we use an array of different techniques for ground-based simulation of microgravity effects to assess the effect of suboptimal environmental conditions on the gene expression of Drosophila in reduced gravity. A global and integrative analysis, using “gene expression dynamics inspector” (GEDI) self-organizing maps, reveals different degrees in the responses of the transcriptome when using different environmental conditions or microgravity/hypergravity simulation devices. Although the genes that are affected are different in each simulation technique, we find that the same gene ontology groups, including at least one large multigene family related with behavior, stress response or organogenesis, are over represented in each case. These results suggest that the transcriptome as a whole can be finely tuned to gravity force. In optimum environmental conditions, the alteration of gravity has only mild effects on gene expression but when environmental conditions are far from optimal, the gene expression must be tuned greatly and effects become more robust, probably linked to the lack of experience of organisms exposed to evolutionary novel environments such as a gravitational free one.
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影响因子：
4.9
作者：
Herranz, Raul;Benguria, Alberto;Marco, Roberto
通讯作者：
Marco, Roberto
影响因子：
5.8
作者：
Eichler, GS;Huang, S;Ingber, DE
通讯作者：
Ingber, DE
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.1
作者：
Benguria, A;Grande, E;Marco, R
通讯作者：
Marco, R
影响因子：
1.8
作者：
Herranz, Raul;Lavan, David A.;Marco, Roberto
通讯作者：
Marco, Roberto