Virome Variation during Sea Star Wasting Disease Progression in Pisaster ochraceus (Asteroidea, Echinodermata).
Virome Variation during Sea Star Wasting Disease Progression in Pisaster ochraceus (Asteroidea, Echinodermata).
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DOI：
10.3390/v12111332
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发表时间：
2020-11-20
期刊：
影响因子：
--
通讯作者：
DeRito CM
中科院分区：
文献类型：
--
作者：
Hewson I;Aquino CA;DeRito CM
海星星消耗病（SSWD）是一种影响小行星超过120年的疾病，但对这种消耗病因的机械理解仍然难以捉摸。我们研究了时间病毒组的变化，在没有外部刺激的情况下浪费的两个Pisaster赭色标本和两个标本，没有经历SSWD的持续时间，我们的研究，并比较病毒组的浪费病变边缘组织的人工瘢痕边缘和大体正常组织随着时间的推移。所有受SSWD影响的组织文库的全局组装导致在>1个文库中表示的24个病毒基因组片段。基因组片段主要匹配浓核病毒和小核糖核酸病毒，匹配的野田病毒较少，还有一种sobemovirus。小核糖核酸病毒样和浓核病毒样基因组片段与其他海洋无脊椎动物宏基因组研究中回收的病毒基因组最相似。Read招募显示，只有两个小核糖核酸病毒样基因组片段仅从受SSWD影响的标本中招募，但这两个片段都不是消耗性病变所独有的。消耗病变边缘读数招募到更多数量的病毒基因型（即，丰富度）比疤痕组织和大体正常组织读数更高。总之，这些数据表明没有单一的病毒基因组片段与SSWD相关。相反，消耗性病变边缘通常可支持病毒增殖。
Sea star wasting disease (SSWD) is a condition that has affected asteroids for over 120 years, yet mechanistic understanding of this wasting etiology remains elusive. We investigated temporal virome variation in two Pisaster ochraceus specimens that wasted in the absence of external stimuli and two specimens that did not experience SSWD for the duration of our study, and compared viromes of wasting lesion margin tissues to both artificial scar margins and grossly normal tissues over time. Global assembly of all SSWD-affected tissue libraries resulted in 24 viral genome fragments represented in >1 library. Genome fragments mostly matched densoviruses and picornaviruses with fewer matching nodaviruses, and a sobemovirus. Picornavirus-like and densovirus-like genome fragments were most similar to viral genomes recovered in metagenomic study of other marine invertebrates. Read recruitment revealed only two picornavirus-like genome fragments that recruited from only SSWD-affected specimens, but neither was unique to wasting lesions. Wasting lesion margin reads recruited to a greater number of viral genotypes (i.e., richness) than did either scar tissue and grossly normal tissue reads. Taken together, these data suggest that no single viral genome fragment was associated with SSWD. Rather, wasting lesion margins may generally support viral proliferation.
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影响因子：
2.6
作者：
Gomez, Dennis K.;Baeck, Gun Wook;Park, Se Chang
通讯作者：
Park, Se Chang
影响因子：
3.8
作者：
Bonami, JR;Hasson, KW;Lightner, DV
通讯作者：
Lightner, DV
影响因子：
3.7
作者：
Grasis, Juris A.;Lachnit, Tim;Bosch, Thomas C. G.
通讯作者：
Bosch, Thomas C. G.
DOI：
10.1098/rstb.2015.0212
发表时间：
2016-03-05
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Eisenlord ME;Groner ML;Yoshioka RM;Elliott J;Maynard J;Fradkin S;Turner M;Pyne K;Rivlin N;van Hooidonk R;Harvell CD
通讯作者：
Harvell CD
影响因子：
5.2
作者：
Debat HJ
通讯作者：
Debat HJ