Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells.
Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells.
复制标题
DOI：
10.1111/j.1582-4934.2009.00765.x
复制
发表时间：
2010-05
影响因子：
5.3
通讯作者：
Sheng Z
中科院分区：
文献类型：
--
作者：
Zhang C;Fu X;Chen P;Bao X;Li F;Sun X;Lei Y;Cai S;Sun T;Sheng Z
分化的表皮细胞在体内可以去分化为干细胞或干细胞样细胞。在这项研究中，我们报告了去分化衍生细胞的分离和表征。将除去基底干细胞的表皮片移植到 47 只裸无胸腺 (BALB/c-nu/nu) 小鼠的皮肤伤口上。 5天后，CK10阴性但CK19和β1-整合素阳性的细胞出现在表皮片的伤口附近一侧。此外，流式细胞分析检测到移植后CK19和β1-整合素+细胞的百分比增加（P < 0.01），而移植片中CK10+细胞的百分比减少（P < 0.01）。然后我们在与IV型胶原快速粘附的基础上分离这些细胞，发现10分钟内移植组中有4.56%的粘附细胞（去分化衍生细胞）。体外表型检测显示，去分化细胞中CK19、β1-integrin、Oct4和Nanog的表达量显着高于对照组（分化表皮细胞）（P < 0.01）。此外，对去分化细胞的功能研究结果表明：（1）与对照相比，由5-10个细胞和大于10个细胞组成的集落数量分别增加了5.9倍和6.7倍（P < 0.01）； (2)更多的细胞处于细胞周期的S期和G2/M期(增殖指数值对照组为21.02%，去分化组为45.08%)； (3) 培养总天数（28 天与 130 天）、细胞传代数（3 代与 20 代）和假设的总细胞产量（1 × 105 个细胞与 1 × 1012 个细胞）均显着增加，（4） 去分化衍生细胞以及表皮干细胞能够再生皮肤等同物，但分化的表皮细胞则不能。这些结果表明体外培养的去分化细胞的特征与表皮干细胞相似。这项研究还可能提供一种产生表皮干细胞用于伤口修复和再生的新方法。
Differentiated epidermal cells can dedifferentiate into stem cells or stem cell-like cells in vivo. In this study, we report the isolation and characterization of dedifferentiation-derived cells. Epidermal sheets eliminated of basal stem cells were transplanted onto the skin wounds in 47 nude athymic (BALB/c-nu/nu) mice. After 5 days, cells negative for CK10 but positive for CK19 and β1-integrin emerged at the wound-neighbouring side of the epidermal sheets. Furthermore, the percentages of CK19 and β1-integrin+ cells detected by flow cytometric analysis were increased after grafting (P < 0.01) and CK10+ cells in grafted sheets decreased (P < 0.01). Then we isolated these cells on the basis of rapid adhesion to type IV collagen and found that there were 4.56% adhering cells (dedifferentiation-derived cells) in the grafting group within 10 min. The in vitro phenotypic assays showed that the expressions of CK19, β1-integrin, Oct4 and Nanog in dedifferentiation-derived cells were remarkably higher than those in the control group (differentiated epidermal cells) (P < 0.01). In addition, the results of the functional investigation of dedifferentiation-derived cells demonstrated: (1) the numbers of colonies consisting of 5–10 cells and greater than 10 cells were increased 5.9-fold and 6.7-fold, respectively, as compared with that in the control (P < 0.01); (2) more cells were in S phase and G2/M phase of the cell cycle (proliferation index values were 21.02% in control group, 45.08% in group of dedifferentiation); (3) the total days of culture (28 days versus 130 days), the passage number of cells (3 passages versus 20 passages) and assumptive total cell output (1 × 105 cells versus 1 × 1012 cells) were all significantly increased and (4) dedifferentiation-derived cells, as well as epidermal stem cells, were capable of regenerating a skin equivalent, but differentiated epidermal cells could not. These results suggested that the characteristics of dedifferentiation-derived cells cultured in vitro were similar to epidermal stem cells. This study may also offer a new approach to yield epidermal stem cells for wound repair and regeneration.
登录
查看更多内容
影响因子：
11.5
作者：
Helm, J;Enkemann, SA;Yeatman, TJ
通讯作者：
Yeatman, TJ
影响因子：
64.5
作者：
Mitsui, K;Tokuzawa, Y;Yamanaka, S
通讯作者：
Yamanaka, S
影响因子：
64.8
作者：
Eggan, E;Baldwin, K;Jaenisch, R
通讯作者：
Jaenisch, R
DOI：
10.1016/j.bbrc.2007.08.155
发表时间：
2007-11-23
影响因子：
3.1
作者：
Kellouche, Sabrina;Martin, Christophe;Dosquet, Christine
通讯作者：
Dosquet, Christine
影响因子：
3.9
作者：
Li, Haihong;Fu, Xiaobing;Wang, Jun
通讯作者：
Wang, Jun