The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies
The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies
复制标题
使用转基因植物了解转座机制并开发转座子标记策略
DOI：
10.1007/bf00023995
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发表时间：
1991
影响因子：
5.1
通讯作者：
J. Hille
中科院分区：
文献类型：
--
作者：
M. Haring;C. Rommens;H. Nijkamp;J. Hille
本文比较了植物转座因子Ac、Tam 3、En/Spm和Mu在异源植物和原宿主中的活性。对自主转座因子和双因子系统的突变分析提供了数据，揭示了转座机制的一些基本性质。通过纯化转座酶蛋白的体外结合研究检测了Ac和En/Spm的功能部分，并测试了它们在异源植物物种中这些转座元件功能中的重要性。已经进行的调节Ac转座因子活性的实验正在进行中，并且已经汇编了初步结果。展望操纵转座因子的转座子标签策略在异源植物物种进行了讨论。
This review compares the activity of the plant transposable elements Ac, Tam3, En/Spm and Mu in heterologous plant species and in their original host. Mutational analysis of the autonomous transposable elements and two-element systems have supplied data that revealed some fundamental properties of the transposition mechanism. Functional parts of Ac and En/Spm were detected by in vitro binding studies of purified transposase protein and have been tested for their importance in the function of these transposable elements in heterologous plant species. Experiments that have been carried out to regulate the activity of the Ac transposable element are in progress and preliminary results have been compiled. Perspectives for manipulated transposable elements in transposon tagging strategies within heterologous plant species are discussed.
DOI：
10.1073/pnas.85.18.6856
发表时间：
1988-09-01
影响因子：
11.1
作者：
CHANG, C;BOWMAN, JL;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM
DOI：
10.1073/pnas.83.5.1408
发表时间：
1986-03-01
影响因子：
11.1
作者：
CHANG, C;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM
Ac 从玉米 P 基因座转座到未复制的染色体位点。
影响因子：
3.3
作者：
Chen,J;Greenblatt,IM;Dellaporta,SL
通讯作者：
Dellaporta,SL