Deuterated hydrazino-s-triazine as highly-efficient labelling reagent for glycan relative quantification analysis using electrospray ionization mass spectrometry
Deuterated hydrazino-s-triazine as highly-efficient labelling reagent for glycan relative quantification analysis using electrospray ionization mass spectrometry
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氘代肼基-s-三嗪作为高效标记试剂，用于使用电喷雾电离质谱法进行聚糖相对定量分析
DOI：
10.1039/c5ra12005e
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发表时间：
2015-09
期刊：
影响因子：
3.9
通讯作者：
Zhang, Xin-Xiang
中科院分区：
文献类型：
--
作者：
Deng, Yan;Zhang, Fang-Ting;Zhou, Ying-Lin;Zhang, Xin-Xiang
本文采用自行设计的非还原性肼基均三嗪氘代衍生物作为标记试剂，结合质谱技术，实现了一种新颖的N-聚糖稳定同位素相对定量策略。高达20 Da的质量位移可以有效区分在还原端用正常和重肼基-s-三嗪标记的不同形式的N-聚糖。特别是对于高分子量的聚糖，由于避免了质谱中同位素分布的重叠，定性鉴定可以显着简化。同时，高纯度的氘衍生物保证了相对定量的准确性。对正常和重肼基-s-三嗪标记的聚糖在电喷雾电离质谱中的明显未分化的响应证实了我们提出的策略的可行性和可靠性，这进一步证明了标记的N-聚糖的混合物从卵白蛋白裂解作为模型样品的相对定量。最后，我们采用人血清作为复杂样本，成功实现了48种N-聚糖的高准确度检测。氘代肼基均三嗪标记试剂在药学和诊断研究中对高效、准确、可靠的N-聚糖相对定量的发展具有巨大的潜力。
Herein, an innovative stable-isotope relative quantification strategy for N-glycans was achieved using a self-designed non-reductive hydrazino-s-triazine deuterated derivative as a labelling reagent combined with mass spectrometry. As much as a 20 Da mass shift could effectively distinguish different forms of N-glycans labelled with normal and heavy hydrazino-s-triazine at the reducing end. Especially for glycans with high molecular weight, qualitative identification could be significantly simplified on account of avoiding isotopic distributions overlapping in the mass spectra. Meanwhile, a deuterium derivative with high purity guaranteed the accuracy of the relative quantification. The obviously undifferentiated response toward normal and heavy hydrazino-s-triazine labelled glycans in electrospray ionization mass spectrometry confirmed the feasibility and reliability of our proposed strategy, which was further demonstrated by relative quantification of the mixtures of labelled N-glycans cleaved from ovalbumin as a model sample. Finally, we adopted human serum as a complex sample and successfully achieved highly accurate detection of 48 N-glycans. The deuterated hydrazino-s-triazine labelling reagent exhibited a great potential to promote the development of highly-efficient, accurate and reliable N-glycan relative quantification in pharmacy and diagnosis research.
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影响因子：
3.1
作者：
Tie, Cai;Zhang, Xin-Xiang
通讯作者：
Zhang, Xin-Xiang
影响因子：
3.5
作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
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