DNA Encapsulation of Ten Silver Atoms Produces a Bright, Modulatable, Near Infrared-Emitting Cluster.
DNA Encapsulation of Ten Silver Atoms Produces a Bright, Modulatable, Near Infrared-Emitting Cluster.
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DOI：
10.1021/jz100817z
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发表时间：
2010-09-02
期刊：
影响因子：
--
通讯作者：
Dickson RM
中科院分区：
文献类型：
--
作者：
Petty JT;Fan C;Story SP;Sengupta B;Iyer AS;Prudowsky Z;Dickson RM
光稳定性、固有荧光亮度和荧光的光学调制是区分银纳米团簇作为荧光团的关键属性。DNA通过保护水环境中的簇和指导它们的形成起着核心作用。在这里，我们描述了一个新的近红外发射集群的激发和发射最大值分别在750和810 nm，是稳定在C3 AC 3AC 3 TC 3A。在近红外物质的色谱解析之后，确定10 Ag/寡核苷酸的化学计量。结合优异的光稳定性，该簇的30%荧光量子产率和180，000 M−1cm−1的消光系数使其荧光亮度显着提高了有机染料Cy 7的荧光亮度。荧光相关分析显示了一个光学上可访问的暗态，可以直接用较长波长的共照明去填充。总荧光的耦合增加表明，通过同步放大荧光图像恢复（SAFIRe）可以实现增强的灵敏度，这进一步区分了这种新的荧光团。
Photostability, inherent fluorescence brightness, and optical modulation of fluorescence are key attributes distinguishing silver nanoclusters as fluorophores. DNA plays a central role both by protecting the clusters in aqueous environments and by directing their formation. Herein, we characterize a new near infrared-emitting cluster with excitation and emission maxima at 750 and 810 nm, respectively that is stabilized within C3AC3AC3TC3A. Following chromatographic resolution of the near infrared species, a stoichiometry of 10 Ag/oligonucleotide was determined. Combined with excellent photostability, the cluster’s 30% fluorescence quantum yield and 180,000 M−1cm−1 extinction coefficient give it a fluorescence brightness that significantly improves on that of the organic dye Cy7. Fluorescence correlation analysis shows an optically accessible dark state that can be directly depopulated with longer wavelength co-illumination. The coupled increase in total fluorescence demonstrates that enhanced sensitivity can be realized through Synchronously Amplified Fluorescence Image Recovery (SAFIRe), which further differentiates this new fluorophore.
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DOI：
10.1126/science.1168683
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Shu X;Royant A;Lin MZ;Aguilera TA;Lev-Ram V;Steinbach PA;Tsien RY
通讯作者：
Tsien RY
影响因子：
6.7
作者：
Pauli, Jutta;Vag, Tibor;Hilger, Ingrid
通讯作者：
Hilger, Ingrid
影响因子：
15
作者：
Richards CI;Hsiang JC;Senapati D;Patel S;Yu J;Vosch T;Dickson RM
通讯作者：
Dickson RM
影响因子：
29.4
作者：
Gwinn, Elisabeth G.;O'Neill, Patrick;Fygenson, Deborah Kuchnir
通讯作者：
Fygenson, Deborah Kuchnir
DOI：
10.1073/pnas.0610677104
发表时间：
2007-07-31
影响因子：
11.1
作者：
Vosch, Tom;Antoku, Yasuko;Dickson, Robert M.
通讯作者：
Dickson, Robert M.