Differential expression of lipoprotein genes in Mycoplasma pneumoniae after contact with human lung epithelial cells, and under oxidative and acidic stress.
Differential expression of lipoprotein genes in Mycoplasma pneumoniae after contact with human lung epithelial cells, and under oxidative and acidic stress.
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DOI：
10.1186/1471-2180-8-124
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发表时间：
2008-07-23
期刊：
影响因子：
4.2
通讯作者：
Browning, Glenn F.
中科院分区：
文献类型：
--
作者：
Hallamaa, Katri M.;Tang, Sen-Lin;Ficorilli, Nino;Browning, Glenn F.
肺炎支原体是一种人类病原体，是社区获得性肺炎的常见原因。它含有大量的脂蛋白基因，其中大多数是未知的功能。由于它们位于细胞表面，这些蛋白质可能参与细菌对环境变化的反应，或参与感染的初始阶段。本研究的目的是确定编码表面脂蛋白的基因是否在与人类细胞系接触后或暴露于氧化或酸性应激后差异表达。利用qRT-PCR分析，我们观察到当M.将肺炎链球菌与人细胞接触。相比之下，脂蛋白表达通常下调或不变时，暴露于过氧化氢或低pH值（5.5）。当暴露于低pH值时，脂蛋白多基因家族6中的四个多顺反子转录基因的mRNA水平随着与预测启动子的距离的增加而形成数量递减的梯度。所展示的转录变化为这些大多数未分配的基因的功能提供了证据，并表明它们响应于环境条件的变化而受到调节。此外，我们已经表明脂蛋白基因家族6的成员可以多顺反子表达。
Mycoplasma pneumoniae is a human pathogen that is a common cause of community-acquired pneumonia. It harbours a large number of lipoprotein genes, most of which are of unknown function. Because of their location on the cell surface, these proteins are likely to be involved in the bacterial response to environmental changes, or in the initial stages of infection. The aim of this study was to determine if genes encoding surface lipoproteins are differentially expressed after contact with a human cell line, or after exposure to oxidative or acidic stress. Using qRT-PCR assays, we observed that the expression of a number of lipoprotein genes was up-regulated when M. pneumoniae was placed in contact with human cells. In contrast, lipoprotein expression was generally down-regulated or unchanged when exposed to either hydrogen peroxide or low pH (5.5). When exposed to low pH, the mRNA levels of four polycistronically transcribed genes in Lipoprotein Multigene Family 6 formed a gradient of decreasing quantity with increasing distance from a predicted promoter. The demonstrated transcriptional changes provide evidence for the functionality of these mostly unassigned genes and indicate that they are regulated in response to changes in environmental conditions. In addition we have shown that the members of Lipoprotein Gene Family 6 may be expressed polycistronically.
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