Influence of diet shift from bloodworm to formulated feed on growth performance, gut microbiota structure and function in early juvenile stages of hybrid sturgeon (Acipenser baerii × Acipenser schrenckii)
Influence of diet shift from bloodworm to formulated feed on growth performance, gut microbiota structure and function in early juvenile stages of hybrid sturgeon (Acipenser baerii × Acipenser schrenckii)
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从红虫到配合饲料的饮食转变对杂交鲟（Acipenser baerii – Acipenser schrenckii）幼鱼早期生长性能、肠道微生物群结构和功能的影响
DOI：
10.1016/j.aquaculture.2020.736165
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发表时间：
2020-11
期刊：
影响因子：
4.5
通讯作者：
Zhigang Zhou
中科院分区：
文献类型：
--
作者：
Qiang Hao;Tsegay Teame;Xuexiang Wu;Qianwen Ding;Chao Ran;Yalin Yang;Yuenan Xing;Zhen Zhang;Zhigang Zhou
目前鲟鱼养殖是我国常见的冷水养殖之一，但与我国冷水鱼养殖潜力相比，其产量很低。鲟鱼早期生活阶段缺乏合适的饲料是造成鲟鱼产量低的主要原因之一。本研究旨在探讨饲料从活饲料（红虫）转换为配合饲料对西伯利亚鲟（Acipenser baerii×Acipenser schrenckii）早期幼鱼生长性能、肠道菌群结构和功能的影响。与红虫组相比，在饲喂配制物的鲟鱼组中观察到增重率（WGR）和饲料转化率（FCR）显著增加（p< 0.05）。在配制饲料组中记录到显著更高的变形菌门（p < 0.05），而在红虫组中梭菌门显著增加（p < 0.05）。此外，使用无菌（GF）斑马鱼模型评估了从每组鲟鱼中分离的肠道微生物群的定殖对宿主免疫的影响。结果表明，饲喂配合饲料后，感染鲟鱼肠道菌群的GF斑马鱼体内抗炎相关基因（IL-10、TGF-β）、非特异性免疫相关基因（溶菌酶、C3 a）和抗病毒相关基因（IFNΦ3、Mxb、Mxc）的表达显著降低，而促炎相关基因（TNFα）的表达显著升高。此外，GF斑马鱼定殖与肠道菌群的鲟鱼喂养的配方饲料显示出显着较低的存活率为A。veroniHm 091攻击（p< 0.05）。因此，与红虫相比，配合饲料提高了鲟鱼的增重率和饲料转化率，但导致肠道菌群失调，从而影响了GF斑马鱼的免疫应答，降低了其对病原菌的抵抗力。
Currently sturgeon farming is one of the common cold fresh water farming in China, but its production is very low comparing with the country's cold water fish farming potential. One of the main reasons for the low production of sturgeon is unavailability of proper feed for early live stage of sturgeon. The aim of this study was to examine the effects of diet shift from live food (bloodworm) to formulated feed on growth performance, structure and function of gut microbiota in early juvenile stages of hybrid sturgeon (Acipenser baerii×Acipenser schrenckii). Significantly increased in weight gain rate (WGR) and feed conversion ratio (FCR) were observed in the sturgeon group fed on the formulated versus the bloodworm group (p< 0.05). Significantly higher Proteobacteria was recorded in the formulated feed group (p < 0.05), while Fusobacteria was significantly increased in the bloodworm group (p < 0.05). Furthermore, effects of colonization of the gut microbiota isolated from each group of sturgeon on host immunity was evaluated using germ free (GF) zebrafish model. The expression of genes related to anti-inflammatory (IL-10, TGF-β), non-specific immune (Lysozyme, C3a), and anti-virus (IFNΦ3, Mxb, Mxc) were significantly decreased in GF zebrafish colonized with gut microbiota of sturgeon fed on formulated feed, while expression of pro-inflammatory gene (TNFα) was significantly increased in formulated feed group. Moreover, GF zebrafish colonized with gut microbiota of sturgeon fed on the formulated feed revealed significantly lower survival rate forA. veroniiHm091 challenge (p< 0.05). In conclusion, the present study revealed that, compared with bloodworm, the formulated feed enhanced the WGR and FCR of sturgeon, but induced intestinal microbiota dysbiosis, which negatively affected the immune response of GF zebrafish and decreased their resistance to pathogenic bacteria.
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DOI：
10.3153/ar18002
发表时间：
2018-01
期刊：
--
影响因子：
--
作者：
G. Zhelyazkov
通讯作者：
G. Zhelyazkov
影响因子：
4
作者：
Pu, ZY;Dobos, M;Powell, IB
通讯作者：
Powell, IB
DOI：
10.1016/j.cbpa.2006.12.037
发表时间：
2007-05-01
影响因子：
2.3
作者：
Kotzamanis, Y. P.;Gisbert, E.;Cahu, C.
通讯作者：
Cahu, C.
影响因子：
3.7
作者：
T. T. Hien-T.;Nguyen Hoang Duc Trung;Bui Minh Tâm;Vo Minh Que Chau;Nguyen Hoang Huy;C. Lee;D. Bengtson
通讯作者：
T. T. Hien-T.;Nguyen Hoang Duc Trung;Bui Minh Tâm;Vo Minh Que Chau;Nguyen Hoang Huy;C. Lee;D. Bengtson
影响因子：
0.6
作者：
V. R. Vartak;Ravendra Singh
通讯作者：
V. R. Vartak;Ravendra Singh