Acidic Electrolyzed Water Inhibits the Viability of Gardnerella spp. via Oxidative Stress Response.
Acidic Electrolyzed Water Inhibits the Viability of Gardnerella spp. via Oxidative Stress Response.
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酸性电解水抑制加德纳菌属的活力。
DOI：
10.3389/fmed.2022.817957
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发表时间：
2022
影响因子：
3.9
通讯作者：
Zhu L
中科院分区：
文献类型：
--
作者：
Zhao C;Chen Y;Gao L;Huang J;Yang X;Pei L;Ye Z;Zhu L
以乳酸杆菌为主的阴道微生物群在维持女性健康方面起着重要作用。阴道微生物群的紊乱允许各种病原体如加德纳菌属（Gardnerella spp.）(GS)和相关的厌氧菌导致细菌性阴道病（BV）。目前，BV的治疗选择非常有限。抗菌药物治疗和阴道酸化是两种主要的治疗方法。酸性电解水（AEW）已知可杀灭微生物，近年来被认为是一种医疗应用。研究发现，嗜酸乳杆菌（LA）益生菌有助于抑制GS诱导的BV。本研究以GS和LA为研究对象，旨在探讨AEW作为BV潜在的替代治疗方法及其机制。我们首先获得AEW的pH值（3.71-4.22）接近正常阴道pH值（3.8-4.5），以维持正常的阴道酸化条件。平板计数实验表明，AEW（pH：4.07，ORP：890.67，ACC：20 ppm）（20 ppm）对GS的抑制作用较好，但对LA的影响较小。通过细胞生物学实验和透射电镜观察，初步探讨了AEW抗GS的可能机制。结果表明，与LA相比，GS处理的AEW明显增加了细胞膜透性，破坏了细胞膜的完整性。AEW还引起GS蛋白质渗漏和细胞溶解，但不影响LA。同时，AEW诱导GS产生大量活性氧（ROS），而LA无明显变化。最后，我们发现20 ppm AEW对经Amsel标准和唾液酸李子法诊断为BV的妇女的阴道分泌物具有极好的抗菌作用。综上所述，我们的研究结果表明，20 ppm AEW对GS具有良好的抗菌效果，对LA的影响较小，有望成为BV的潜在治疗方法。
The vaginal microbiota, dominated by Lactobacilli, plays an important role in maintaining women's health. Disturbance of the vaginal microbiota allows infection by various pathogens such as Gardnerella spp. (GS) and related anaerobic bacteria resulting in bacterial vaginosis (BV). At present, the treatment options for BV are extremely limited. Treatment of antibacterial drugs and vaginal acidification are the two primary therapeutic methods. Acid electrolyzed water (AEW) is known to inactivate microorganisms and is considered a medical application in recent years. Studies have found that Lactobacillus acidophilus (LA) probiotics helps to inhibit GS-induced BV. Our study took GS and LA as the research object, which aims to explore AEW as a potential alternative therapy for BV and its underlying mechanisms. We first obtained the pH of AEW (3.71–4.22) close to normal vaginal pH (3.8–4.5) to maintain normal vaginal acidification conditions. Plate counting experiments showed that AEW (pH: 4.07, ORP: 890.67, ACC: 20 ppm) (20 ppm) could better inhibit the viability of GS but had a more negligible effect on LA. Then, we preliminarily explored the possible mechanism of AEW anti-GS using cell biology experiments and transmission electron microscopy. Results showed that the membrane permeability was significantly increased and the integrity of cell membrane was destroyed by AEW in GS than those in LA. AEW also caused protein leakage and cell lysis in GS without affecting LA. Meanwhile, AEW induced a number of reactive oxygen species (ROS) production in GS, with no obvious LA changes. Finally, we found that 20 ppm AEW exhibited excellent antibacterial effect on the vaginal secretions of women diagnosed with BV by Amsel criteria and sialic acid plum method. Taken together, our findings manifest that 20 ppm AEW has an excellent antibacterial effect in GS with less effect on LA, which might be expected to become a potential therapy for BV.
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影响因子：
3.7
作者：
Chavoustie SE;Jacobs M;Reisman HA;Waldbaum AS;Levy SF;Hillier SL;Nyirjesy P
通讯作者：
Nyirjesy P
DOI：
10.1007/s00405-021-06644-5
发表时间：
2021-08
期刊：
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
影响因子：
--
作者：
Giarratana N;Rajan B;Kamala K;Mendenhall M;Reiner G
通讯作者：
Reiner G
影响因子：
5.5
作者：
Liao, Long B.;Chen, Wei M.;Xiao, Xian M.
通讯作者：
Xiao, Xian M.
细菌性阴道病生物膜：对当前疗法和新兴溶液的挑战。
影响因子：
5.2
作者：
Machado D;Castro J;Palmeira-de-Oliveira A;Martinez-de-Oliveira J;Cerca N
通讯作者：
Cerca N
影响因子：
4.4
作者：
Ling Z;Kong J;Liu F;Zhu H;Chen X;Wang Y;Li L;Nelson KE;Xia Y;Xiang C
通讯作者：
Xiang C