Molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis.
Molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis.
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DOI：
10.1186/1471-2164-11-488
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发表时间：
2010-09-07
期刊：
影响因子：
4.4
通讯作者：
Xiang C
中科院分区：
文献类型：
--
作者：
Ling Z;Kong J;Liu F;Zhu H;Chen X;Wang Y;Li L;Nelson KE;Xia Y;Xiang C
细菌性阴道病（BV）是一种阴道微生物群的生态失调，每年影响数百万妇女，并与许多不良健康后果有关，包括早产和性传播感染的获得。然而，对阴道微生物群落的整体结构和组成知之甚少;大多数早期研究集中在BV过程中的主要阴道细菌。本研究采用非培养PCR-变性梯度凝胶电泳（DGGE）和条形码454焦磷酸测序方法，研究了来自中国的50名BV阳性女性和50名健康女性阴道微生物群的多样性和丰富性，并通过定量PCR进行验证。我们的数据表明，当比较与健康和疾病状况相关的人群时，阴道中存在的细菌种类的绝对和相对丰度发生了深刻的变化。尽管存在显著的人际差异，但两组中阴道微生物群的多样性可以清楚地分为两个集群。共获得246，359个高质量焦磷酸测序读数用于评估细菌多样性，24，298个独特序列代表所有细菌类型。在阴道中鉴定的最主要的细菌门属于厚壁菌门、拟杆菌门、放线菌门和梭菌门。BV阳性女性中的HBV型别数量高于健康女性，这与先前的研究结果一致，并且揭示了大量在先前研究中遗漏的低丰度分类群。虽然没有单一细菌可以被鉴定为健康超过疾病状况的特异性标志物，但是三门-拟杆菌门、放线菌门和梭杆菌门，以及八个属，包括加德纳菌属、奇异菌属、巨球菌属、埃格塞菌属、气球菌属、细毛菌属/Sneathia、普雷沃菌属和乳头状杆菌属，与BV强烈相关（p < 0.05）。这些属是潜在的优良标志物，可作为临床BV分子诊断的靶点。这里提供的数据清楚地描述了阴道群落的整体结构，并清楚地表明BV与阴道微生物群的分类丰富性和多样性的急剧增加有关。该研究还提供了阴道群落结构和细菌生态系统的最全面的图片，并显着有助于目前对BV病因的理解。
Bacterial vaginosis (BV) is an ecological disorder of the vaginal microbiota that affects millions of women annually, and is associated with numerous adverse health outcomes including pre-term birth and the acquisition of sexually transmitted infections. However, little is known about the overall structure and composition of vaginal microbial communities; most of the earlier studies focused on predominant vaginal bacteria in the process of BV. In the present study, the diversity and richness of vaginal microbiota in 50 BV positive and 50 healthy women from China were investigated using culture-independent PCR-denaturing gradient gel electrophoresis (DGGE) and barcoded 454 pyrosequencing methods, and validated by quantitative PCR. Our data demonstrated that there was a profound shift in the absolute and relative abundances of bacterial species present in the vagina when comparing populations associated with healthy and diseased conditions. In spite of significant interpersonal variations, the diversity of vaginal microbiota in the two groups could be clearly divided into two clusters. A total of 246,359 high quality pyrosequencing reads was obtained for evaluating bacterial diversity and 24,298 unique sequences represented all phylotypes. The most predominant phyla of bacteria identified in the vagina belonged to Firmicutes, Bacteroidetes, Actinobacteria and Fusobacteria. The higher number of phylotypes in BV positive women over healthy is consistent with the results of previous studies and a large number of low-abundance taxa which were missed in previous studies were revealed. Although no single bacterium could be identified as a specific marker for healthy over diseased conditions, three phyla - Bacteroidetes, Actinobacteria and Fusobacteria, and eight genera including Gardnerella, Atopobium, Megasphaera, Eggerthella, Aerococcus, Leptotrichia/Sneathia, Prevotella and Papillibacter were strongly associated with BV (p < 0.05). These genera are potentially excellent markers and could be used as targets for clinical BV diagnosis by molecular approaches. The data presented here have clearly profiled the overall structure of vaginal communities and clearly demonstrated that BV is associated with a dramatic increase in the taxonomic richness and diversity of vaginal microbiota. The study also provides the most comprehensive picture of the vaginal community structure and the bacterial ecosystem, and significantly contributes to the current understanding of the etiology of BV.
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影响因子：
2.6
作者：
Farage, Miranda;Maibach, Howard
通讯作者：
Maibach, Howard
影响因子：
11.8
作者：
Haggerty, CL;Hillier, SL;Ness, RB
通讯作者：
Ness, RB
DOI：
10.1073/pnas.0807920105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Fierer, Noah;Hamady, Micah;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
影响因子：
6.1
作者：
Boskey, ER;Cone, RA;Moench, TR
通讯作者：
Moench, TR