Identification of Candidate Genes Associated with Trichothecene Biosynthesis in Fusarium graminearum Species Complex Combined with Transcriptomic and Proteomic Analysis.
Identification of Candidate Genes Associated with Trichothecene Biosynthesis in Fusarium graminearum Species Complex Combined with Transcriptomic and Proteomic Analysis.
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结合转录组学和蛋白质组学分析鉴定禾谷镰刀菌复合体中与单端孢菌素生物合成相关的候选基因
DOI：
10.3390/microorganisms10081479
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发表时间：
2022-07-22
期刊：
影响因子：
4.5
通讯作者：
Wu, Aibo
中科院分区：
文献类型：
--
作者：
Wang, Jianhua;Lin, Shanhai;Zhu, Guanghui;Zhao, Zhiyong;Wang, Haoyu;Zhou, Miaoping;Zhao, Xingming;Wu, Aibo
禾谷镰刀菌（Fusarium graminearum）种复合体是世界范围内小麦赤霉病的主要致病因子。单端孢霉烯类在受感染谷物中产生，具有重要的食品安全意义。以往报道的关于镰孢菌产孢的研究都集中在有利于真菌毒素产生的条件上，而镰孢菌菌株在正常或非诱导条件下生物合成镰孢菌烯的分子机制仍不清楚。在这里，一个全球性的分析真菌基因表达的三个菌株使用的Affyelium镰刀菌基因芯片在非诱导条件下的报告。通过生物信息学分析，发现了不同产孢能力菌株间的差异表达基因，并发现了一些与产孢能力相关的新基因。为了验证转录组结果，在相同的培养条件下对三种菌株进行蛋白质组学分析。总共在77个蛋白质点中鉴定出69个独特的真菌蛋白质。结合转录组和蛋白质组分析，预测了27个新基因与地衣芽孢杆菌毒素的产生有关。通过蛋白质组学分析，发现由FGSG_01403编码的蛋白质与二萜烯的产生相关。FGSG_01403的基因敲除突变导致具有增加的多烯产量的突变体。在这项研究中确定的候选基因的未来功能分析可能会揭示新的见解禾谷镰刀菌物种复合体中的烯生产的负调控。
The Fusarium graminearum species complex is the main causal agent of wheat head blight worldwide. Trichothecenes produced by the pathogen in infected grains have important food safety implications. Previously reported studies on trichothecene production have all focused on the conditions conducive to mycotoxin production, while the molecular mechanisms of trichothecene biosynthesis in Fusarium strains under normal or non-inducing conditions are still unclear. Here, a global analysis of the fungal gene expression of three strains using the Affymetrix Fusarium GeneChip under non-inducing conditions is reported. Differentially expressed genes were identified among strains with different trichothecene-production ability, and some novel genes associated with trichothecene biosynthesis were found by bioinformatics analysis. To verify the transcriptome results, proteomic analyses of the three strains were conducted under the same culture conditions. In total, 69 unique fungal proteins were identified in 77 protein spots. Combined with transcriptome and proteome analysis, 27 novel genes were predicted to be associated with trichothecene mycotoxin production. A protein, encoded by FGSG_01403, was found to be associated with trichothecene production via proteome analysis. Gene knock-out mutations of FGSG_01403 resulted in mutants with increased production of trichothecenes. Future functional analysis of the candidate genes identified in this study may reveal new insights into the negative regulation of trichothecene production in the Fusarium graminearum species complex.
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影响因子：
2.9
作者：
Talas F;Würschum T;Reif JC;Parzies HK;Miedaner T
通讯作者：
Miedaner T
影响因子：
4.9
作者：
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作者：
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影响因子：
2.5
作者：
Jenczmionka, NJ;Schäfer, W
通讯作者：
Schäfer, W