Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria.
Production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria.
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通过人体肠道细菌生物转化脱脂亚麻籽生产肠二醇
DOI：
10.1186/1471-2180-10-115
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发表时间：
2010-04-16
期刊：
影响因子：
4.2
通讯作者：
Liu SL
中科院分区：
文献类型：
--
作者：
Wang CZ;Ma XQ;Yang DH;Guo ZR;Liu GR;Zhao GX;Tang J;Zhang YN;Ma M;Cai SQ;Ku BS;Liu SL
肠脂质，如肠二醇（END）及其氧化产物肠内酯（ENL）在预防激素依赖性疾病（如骨质疏松症、心血管疾病、高脂血症、乳腺癌、结肠癌、前列腺癌和更年期综合征）方面的作用已引起人们的广泛关注。迄今为止，获得END和ENL的主要途径是化学合成，这是昂贵的，不可避免地会导致环境污染。为了探索一种更经济、更环保的生产方法，我们探索了由脱脂亚麻籽中含有的前体在人体肠道细菌中的生物转化。结果健康青壮年粪便标本在脱脂亚麻籽培养基中培养，培养上清中检测到END。通过以脱脂亚麻籽为唯一碳源的粪便微生物群的连续传代培养，我们获得了一个细菌联合体，命名为END-49，其中包含的细菌类型数量最少，仍然能够代谢脱脂亚麻籽产生END。基于脉冲场凝胶电泳分析，发现END-49由5个基因组不同的细菌谱系组成，称为I- v群，其中I群菌株在培养中占主导地位。没有一株I-V组菌株产生END，这表明脱脂亚麻籽中的底物转化为END是END-49细菌联合体不同成员的共同工作。有趣的是，第一组菌株产生第二异脂树脂醇，这是生产END的重要中间体；1株I群菌株的16S rRNA分析证实其与克雷伯菌亲缘关系较近。基因组分析正在进行中，以确定所有参与生物转化的END-49成员和导致end -生产的实际途径。结论以脱脂亚麻籽为原料，生物转化是一种经济、高效、环保的大规模生产肠二醇的方法。
BackgroundThe effects of enterolignans, e.g., enterodiol (END) and particularly its oxidation product, enterolactone (ENL), on prevention of hormone-dependent diseases, such as osteoporosis, cardiovascular diseases, hyperlipemia, breast cancer, colon cancer, prostate cancer and menopausal syndrome, have attracted much attention. To date, the main way to obtain END and ENL is chemical synthesis, which is expensive and inevitably leads to environmental pollution. To explore a more economic and eco-friendly production method, we explored biotransformation of enterolignans from precursors contained in defatted flaxseeds by human intestinal bacteria.ResultsWe cultured fecal specimens from healthy young adults in media containing defatted flaxseeds and detected END from the culture supernatant. Following selection through successive subcultures of the fecal microbiota with defatted flaxseeds as the only carbon source, we obtained a bacterial consortium, designated as END-49, which contained the smallest number of bacterial types still capable of metabolizing defatted flaxseeds to produce END. Based on analysis with pulsed field gel electrophoresis, END-49 was found to consist of five genomically distinct bacterial lineages, designated Group I-V, with Group I strains dominating the culture. None of the individual Group I-V strains produced END, demonstrating that the biotransformation of substrates in defatted flaxseeds into END is a joint work by different members of the END-49 bacterial consortium. Interestingly, Group I strains produced secoisolariciresinol, an important intermediate of END production; 16S rRNA analysis of one Group I strain established its close relatedness withKlebsiella. Genomic analysis is under way to identify all members in END-49 involved in the biotransformation and the actual pathway leading to END-production.ConclusionBiotransformation is a very economic, efficient and environmentally friendly way of mass-producing enterodiol from defatted flaxseeds.
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影响因子：
7.2
作者：
Lemay, A;Dodin, S;Forest, JC
通讯作者：
Forest, JC
影响因子：
6.1
作者：
Heinonen, S;Nurmi, T;Adlercreutz, H
通讯作者：
Adlercreutz, H
影响因子：
4.3
作者：
Kitts, DD;Yuan, YV;Thompson, LU
通讯作者：
Thompson, LU
影响因子：
64.8
作者：
AXELSON, M;SJOVALL, J;SETCHELL, KDR
通讯作者：
SETCHELL, KDR
影响因子：
2
作者：
Jin, Jong-Sik;Zhao, Yu-Feng;Hattori, Masao
通讯作者：
Hattori, Masao