Trimethylamine N-Oxide Response to a Mixed Macronutrient Tolerance Test in a Cohort of Healthy United States Adults.
Trimethylamine N-Oxide Response to a Mixed Macronutrient Tolerance Test in a Cohort of Healthy United States Adults.
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DOI：
10.3390/ijms24032074
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发表时间：
2023-01-20
影响因子：
5.6
通讯作者：
Bennett, Brian J.
中科院分区：
文献类型：
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作者：
James, Kristen L.;Gertz, Erik R.;Kirschke, Catherine P.;Allayee, Hooman;Huang, Liping;Kable, Mary E.;Newman, John W.;Stephensen, Charles B.;Bennett, Brian J.
关键词：
饲喂三甲胺、胆碱、磷脂酰胆碱、L肉碱和甜菜碱后，血浆三甲胺氮氧化物(TMAO)浓度升高，但尚不清楚在有限数量的TMAO前体进行混合常量营养素耐量试验后，浓度是否会发生变化。在这项概念验证研究中，我们为年龄(18-65岁)和体重指数(18-44 kg/m2)的健康成年女性和男性(n=97)提供了MMTT(60%脂肪，25%蔗糖；42%标准2000公斤卡路里饮食)，并记录了他们在空腹、餐后30分钟、3小时和6小时的代谢反应。我们量化了总接触TMAO(AUC-TMAO)，并根据他们经历最大TMAO浓度的抽血情况对个体进行了分类(TMAO反应组)。我们将AUC-TMAO与16S rRNA微生物组、FMO3基因外显子上的两个SNP(rs2266782，G>；A，p.Glu158Lys；和rs2266780，A>；G，p.Glu308Gly)以及先验的血浆代谢物相关联。我们观察到不同的TMAO反应(时间和幅度)，并通过年龄交互作用识别性别，使得女性的AUC-TMAO随着年龄的增长而增加，但在男性中没有增加(p值=0.0112)。AUC-TMAO与粪便微生物组和FMO3基因之间的关系不明显。我们观察到AUC-TMAO与肿瘤坏死因子-α之间有很强的相关性，且这种相关性依赖于TMAO反应组。这些发现促进了精确营养，并对成年人的饮食行为产生了重要的影响，这些成年人可以从减少TMAO暴露中受益，并有助于了解产生血浆TMAO的因素。
Plasma trimethylamine n-oxide (TMAO) concentration increases in responses to feeding TMAO, choline, phosphatidylcholine, L-carnitine, and betaine but it is unknown whether concentrations change following a mixed macronutrient tolerance test (MMTT) with limited amounts of TMAO precursors. In this proof-of-concept study, we provided healthy female and male adults (n = 97) ranging in age (18–65 years) and BMI (18–44 kg/m2) a MMTT (60% fat, 25% sucrose; 42% of a standard 2000 kilo calorie diet) and recorded their metabolic response at fasting and at 30 min, 3 h, and 6 h postprandially. We quantified total exposure to TMAO (AUC-TMAO) and classified individuals by the blood draw at which they experienced their maximal TMAO concentration (TMAO-response groups). We related AUC-TMAO to the 16S rRNA microbiome, to two SNPs in the exons of the FMO3 gene (rs2266782, G>A, p.Glu158Lys; and rs2266780, A>G, p.Glu308Gly), and to a priori plasma metabolites. We observed varying TMAO responses (timing and magnitude) and identified a sex by age interaction such that AUC-TMAO increased with age in females but not in males (p-value = 0.0112). Few relationships between AUC-TMAO and the fecal microbiome and FMO3 genotype were identified. We observed a strong correlation between AUC-TMAO and TNF-α that depended on TMAO-response group. These findings promote precision nutrition and have important ramifications for the eating behavior of adults who could benefit from reducing TMAO exposure, and for understanding factors that generate plasma TMAO.
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影响因子：
5.9
作者：
通讯作者：
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卡路里限制对基因表达中昼夜节律的影响是性别依赖性的。
影响因子：
4.6
作者：
Astafev AA;Patel SA;Kondratov RV
通讯作者：
Kondratov RV
影响因子：
9.8
作者：
Schadt EE;Molony C;Chudin E;Hao K;Yang X;Lum PY;Kasarskis A;Zhang B;Wang S;Suver C;Zhu J;Millstein J;Sieberts S;Lamb J;GuhaThakurta D;Derry J;Storey JD;Avila-Campillo I;Kruger MJ;Johnson JM;Rohl CA;van Nas A;Mehrabian M;Drake TA;Lusis AJ;Smith RC;Guengerich FP;Strom SC;Schuetz E;Rushmore TH;Ulrich R
通讯作者：
Ulrich R
影响因子：
4
作者：
Falconi CA;Junho CVDC;Fogaça-Ruiz F;Vernier ICS;da Cunha RS;Stinghen AEM;Carneiro-Ramos MS
通讯作者：
Carneiro-Ramos MS
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP