Microbial interaction between the succinate-utilizing bacterium Phascolarctobacterium faecium and the gut commensal Bacteroides thetaiotaomicron.
Microbial interaction between the succinate-utilizing bacterium Phascolarctobacterium faecium and the gut commensal Bacteroides thetaiotaomicron.
复制标题
DOI：
10.1002/mbo3.1111
复制
发表时间：
2020-10
期刊：
影响因子：
3.4
通讯作者：
Sakamoto M
中科院分区：
文献类型：
--
作者：
Ikeyama N;Murakami T;Toyoda A;Mori H;Iino T;Ohkuma M;Sakamoto M
人类肠道中存在着各种各样的微生物，其中一些被认为是相互作用的。这些相互作用大多涉及细菌代谢物。屎相弧菌几乎不使用碳水化合物生长，而是使用琥珀酸作为底物。本研究研究了琥珀酸专一性利用菌P.faecium与琥珀酸产肠道共生类杆菌thetotaomicron共培养的生长行为。B.thetaiotaomicron的琥珀酸生产支持了屎疫霉的生长，并通过琥珀酸途径伴随丙酸的产生。生产的丁二酸酯完全转化为丙酸酯。这一结果与粪便假单胞菌在添加1%(w/v)琥珀酸的培养液中的培养结果相当。我们分析了粪便假单胞菌和B.thetaiotaomicron单独培养和混合培养之间的转录反应(RNA-Seq)。通过比较单独培养和混合培养条件下屎假单胞菌基因的表达水平，发现与琥珀酸转运有关的基因在混合培养条件下显著表达。差异表达分析表明，屎假单胞菌的存在诱导了B.thaiotaomicron转录模式的变化，例如维生素B12转运蛋白基因的表达变化和谷氨酸依赖的耐酸系统相关基因的表达减少。此外，转录组分析表明，谷氨酸和琥珀酸可能是琥珀酸途径中间产物琥珀酰辅酶A的来源。这项研究揭示了一些糖解菌在人体肠道中的生存策略。类杆菌的琥珀酸生产支持了屎相弧菌的生长，并通过琥珀酸途径伴随丙酸的产生。我们分析了这两个物种的单一培养和共培养之间的转录反应。在屎假单胞菌中，与琥珀酸运输有关的基因在共培养过程中显著表达。转录组分析表明，谷氨酸和琥珀酸可能是琥珀酸途径中间产物琥珀酰辅酶A的来源。
A large variety of microbes are present in the human gut, some of which are considered to interact with each other. Most of these interactions involve bacterial metabolites. Phascolarctobacterium faecium hardly uses carbohydrates for growth and instead uses succinate as a substrate. This study investigated the growth behavior of the co‐culture of the succinate‐specific utilizer P. faecium and the succinogenic gut commensal Bacteroides thetaiotaomicron. Succinate production by B. thetaiotaomicron supported the growth of P. faecium and concomitant propionate production via the succinate pathway. The succinate produced was completely converted to propionate. This result was comparable with the monoculture of P. faecium in the medium supplemented with 1% (w/v) succinate. We analyzed the transcriptional response (RNA‐Seq) between the mono‐ and co‐culture of P. faecium and B. thetaiotaomicron. Comparison of the expression levels of genes of P. faecium between the mono‐ and co‐cultured conditions highlighted that the genes putatively involved in the transportation of succinate were notably expressed under the co‐cultured conditions. Differential expression analysis showed that the presence of P. faecium induced changes in the B. thetaiotaomicron transcriptional pattern, for example, expression changes in the genes for vitamin B12 transporters and reduced expression of glutamate‐dependent acid resistance system‐related genes. Also, transcriptome analysis of P. faecium suggested that glutamate and succinate might be used as sources of succinyl‐CoA, an intermediate in the succinate pathway. This study revealed some survival strategies of asaccharolytic bacteria, such as Phascolarctobacterium spp., in the human gut. Succinate production by Bacteroides thetaiotaomicron supported the growth of Phascolarctobacterium faecium and concomitant propionate production via the succinate pathway. We analyzed the transcriptional response between mono‐ and co‐cultures of these two species. In P. faecium, the genes putatively involved in the transportation of succinate were notably expressed during co‐culture. Transcriptome analysis of P. faecium suggested that glutamate and succinate might be used as sources of succinyl‐CoA, an intermediate in the succinate pathway.
登录
查看更多内容
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
3.7
作者：
Lecomte V;Kaakoush NO;Maloney CA;Raipuria M;Huinao KD;Mitchell HM;Morris MJ
通讯作者：
Morris MJ
DOI：
10.1007/s10482-018-1040-x
发表时间：
2018-06
期刊：
Antonie van Leeuwenhoek
影响因子：
--
作者：
Chia LW;Hornung BVH;Aalvink S;Schaap PJ;de Vos WM;Knol J;Belzer C
通讯作者：
Belzer C
影响因子：
3.2
作者：
FOSTER, JW;HALL, HK
通讯作者：
HALL, HK
影响因子：
2.8
作者：
CASPARI, D;MACY, JM
通讯作者：
MACY, JM