Deciphering the trophic interaction between Akkermansia muciniphila and the butyrogenic gut commensal Anaerostipes caccae using a metatranscriptomic approach.
Deciphering the trophic interaction between Akkermansia muciniphila and the butyrogenic gut commensal Anaerostipes caccae using a metatranscriptomic approach.
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DOI：
10.1007/s10482-018-1040-x
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Belzer C
中科院分区：
文献类型：
--
作者：
Chia LW;Hornung BVH;Aalvink S;Schaap PJ;de Vos WM;Knol J;Belzer C
宿主聚糖在调节人类与其肠道细菌之间的共生关系方面至关重要。宿主分泌的粘蛋白在粘膜层的恒定流量为细菌定植创造了稳定的生态位。关键物种的粘蛋白降解随后形成微生物群落。本研究调查了在粘蛋白驱动的营养相互作用的专门的粘蛋白降解剂嗜粘蛋白阿克曼氏菌和产丁酸的肠道嗜粪Anaerostipes caccae之间的转录反应。A. muciniphila单培养物和与非粘液溶解A.来自毛螺菌科的仙人掌在补充有粘蛋白的基本培养基中厌氧生长。我们分析了生长、代谢物（HPLC分析）、微生物组成（定量逆转录PCR）和转录反应（RNA-seq）。粘蛋白降解A.嗜粘蛋白菌支持A. caccae和伴随的丁酸生产主要通过乙酰辅酶A途径。差异表达分析（DESeq 2）显示A. caccae引起A.结果显示，在嗜粘蛋白的转录应答中，粘蛋白降解基因的表达增加，核糖体基因的表达减少。两个假定的操纵子编码的未表征的蛋白质和外排系统，和几个双组分系统也差异调节。这表明A。muciniphila在A. [咒语]这项研究提供了深入了解粘蛋白驱动的微生物生态学使用元转录组学。我们的研究结果表明，A.嗜粘蛋白菌在群体成员存在时增加。这可能表明其作为关键物种的作用，通过增加粘蛋白糖的可用性来支持粘膜环境中的微生物群落。本文的在线版本（10.1007/s10482-018-1040-x）包含补充材料，可供授权用户使用。
Host glycans are paramount in regulating the symbiotic relationship between humans and their gut bacteria. The constant flux of host-secreted mucin at the mucosal layer creates a steady niche for bacterial colonization. Mucin degradation by keystone species subsequently shapes the microbial community. This study investigated the transcriptional response during mucin-driven trophic interaction between the specialised mucin-degrader Akkermansia muciniphila and a butyrogenic gut commensal Anaerostipes caccae. A. muciniphila monocultures and co-cultures with non-mucolytic A. caccae from the Lachnospiraceae family were grown anaerobically in minimal media supplemented with mucin. We analysed for growth, metabolites (HPLC analysis), microbial composition (quantitative reverse transcription PCR), and transcriptional response (RNA-seq). Mucin degradation by A. muciniphila supported the growth of A. caccae and concomitant butyrate production predominantly via the acetyl-CoA pathway. Differential expression analysis (DESeq 2) showed the presence of A. caccae induced changes in the A. muciniphila transcriptional response with increased expression of mucin degradation genes and reduced expression of ribosomal genes. Two putative operons that encode for uncharacterised proteins and an efflux system, and several two-component systems were also differentially regulated. This indicated A. muciniphila changed its transcriptional regulation in response to A. caccae. This study provides insight to understand the mucin-driven microbial ecology using metatranscriptomics. Our findings show that the expression of mucolytic enzymes by A. muciniphila increases upon the presence of a community member. This could indicate its role as a keystone species that supports the microbial community in the mucosal environment by increasing the availability of mucin sugars. The online version of this article (10.1007/s10482-018-1040-x) contains supplementary material, which is available to authorized users.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
聚糖代谢如何塑造人类肠道微生物群。
DOI：
10.1038/nrmicro2746
发表时间：
2012-04-11
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.8
作者：
de Vos, Willem M.
通讯作者：
de Vos, Willem M.
影响因子：
24.5
作者：
Grander, Christoph;Adolph, Timon E.;Tilg, Herbert
通讯作者：
Tilg, Herbert
DOI：
10.1073/pnas.1219451110
发表时间：
2013-05-28
影响因子：
11.1
作者：
Everard, Amandine;Belzer, Clara;Cani, Patrice D.
通讯作者：
Cani, Patrice D.