Re-Evaluation of Rat Corneal Damage by Short-Wavelength UV Revealed Extremely Less Hazardous Property of Far-UV-C(†).
Re-Evaluation of Rat Corneal Damage by Short-Wavelength UV Revealed Extremely Less Hazardous Property of Far-UV-C(†).
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DOI：
10.1111/php.13419
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发表时间：
2021-05
影响因子：
3.3
通讯作者：
Tanito M
中科院分区：
文献类型：
--
作者：
Kaidzu S;Sugihara K;Sasaki M;Nishiaki A;Ohashi H;Igarashi T;Tanito M
观察了不同波长紫外线（311、254、235、222和207 nm）对大鼠角膜损伤的影响。对于207和222‐UV‐C，阈值辐射暴露在207 nm和222 nm之间，分别为10000 ~ 15000 mJ cm−2和3500 ~ 5000 mJ cm−2。照射后立即通过环丁烯嘧啶二聚体（CPD）定位显示角膜穿透深度与波长有关。311和254 nm的UV可穿透角膜内皮，235 nm的UVC可穿透角膜基质的中间部分，222和207 nm的UVC仅能穿透最外层的角膜上皮。222 nm UVC照射后12 h，角膜上皮CPD消失。短波UV - C诱导角膜损伤的最小剂量远远高于美国政府工业卫生学家会议（ACGIH）颁布的阈值（TLV®）。207和222 nm的UV - C辐射比较长波长的紫外线危害小的原因是，这种辐射只穿透到角膜上皮的最外层。在生理周转周期中，这些细胞通常在24小时内脱落。因此，短波长的UV - C对角膜的危害可能比之前认为的要小。比较本研究最低观察到的不良反应水平（LOAEL）、无观察到的不良反应水平（NOAEL）和美国政府工业卫生学家会议（ACGIH）规定的阈值（TLV®）。辐射暴露阈值估计在LOAEL和NOAEL之间；然而，在远紫外- C波段，如207nm和222nm，与TLV®有很大的区别。我们的结果表明，远紫外- C波段的TLV®可能比以前认为的要高得多。
Corneal damage‐induced various wavelength UV (311, 254, 235, 222 and 207 nm) was evaluated in rats. For 207 and 222‐UV‐C, the threshold radiant exposure was between 10 000 and 15 000 mJ cm−2 at 207 nm and between 3500 and 5000 mJ cm−2 at 222 nm. Penetrate depth to the cornea indicated by cyclobutene pyrimidine dimer (CPD) localization immediately after irradiation was dependent on the wavelength. 311 and 254 nm UV penetrate to corneal endothelium, 235 nm UVC to the intermediate part of corneal stroma, 222 and 207 nm UVC only to the most outer layer of corneal epithelium. CPD observed in corneal epithelium irradiated by 222 nm UVC disappeared until 12 h after. The minimum dose to induce corneal damage of short‐wavelength UV‐C was considerably higher than the threshold limit value (TLV®) promulgated by American Conference of Governmental Industrial Hygienists (ACGIH). The property that explains why UV‐C radiation at 207 and 222 nm is extremely less hazardous than longer UV wavelengths is the fact that this radiation only penetrates to the outermost layer of the corneal epithelium. These cells typically peel off within 24 h during the physiological turnover cycle. Hence, short‐wavelength UV‐C might be less hazardous to the cornea than previously considered until today. Comparison of the lowest observed adverse effects level (LOAEL), the no observed adverse effect level (NOAEL) in this study and the threshold limit value (TLV®) regulated by The American Conference of Governmental Industrial Hygienists (ACGIH). Radiant exposure threshold is estimated to be between LOAEL and NOAEL; however, there is a large difference from TLV® at the far UV‐C band, such as 207 nm and 222 nm. Our results suggested that the TLV® of the far UV‐C band may be much higher than previously thought.
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影响因子：
3.3
作者：
Kaidzu, Sachiko;Sugihara, Kazunobu;Tanito, Masaki
通讯作者：
Tanito, Masaki
影响因子：
4.4
作者：
Tanito, M;Takanashi, T;Ohira, A
通讯作者：
Ohira, A
影响因子：
2.3
作者：
Diffey, Brian L
通讯作者：
Diffey, Brian L
影响因子：
4.6
作者：
Buonanno, Manuela;Welch, David;Brenner, David J.
通讯作者：
Brenner, David J.
DOI：
10.1080/0002889718506444
发表时间：
1971-01-01
期刊：
AMERICAN INDUSTRIAL HYGIENE ASSOCIATION JOURNAL
影响因子：
--
作者：
PITTS, DG;TREDICI, TJ
通讯作者：
TREDICI, TJ