Pre-clinical whole-body fluorescence imaging: Review of instruments, methods and applications.
Pre-clinical whole-body fluorescence imaging: Review of instruments, methods and applications.
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DOI：
10.1016/j.jphotobiol.2009.11.007
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发表时间：
2010-01-21
影响因子：
5.4
通讯作者：
Pogue, Brian W.
中科院分区：
文献类型：
--
作者：
Leblond, Frederic;Davis, Scott C.;Valdes, Pablo A.;Pogue, Brian W.
细胞功能的荧光采样在生物学的各个方面被广泛应用，它能够使细胞和亚细胞生物过程可视化，空间分辨率范围从纳米到厘米不等。体内荧光成像已成为所有临床前工作中最常用的放射学工具。在过去十年中，全身临床前成像系统已经出现，具有广泛的用途和特定的应用领域。能在电磁光谱的可见光到近红外部分被激发的荧光探针的范围不断扩大，由于光的吸收急剧下降，对体内使用最有价值的是波长超过630纳米的部分。全身体内荧光成像尚未达到能在大规模临床前研究中常规使用的成熟状态。这部分是由于对这种成像方式的实际基本能力和局限性缺乏完整的了解。然而，研究实验室不断取得进展，突破该方法的局限，在空间分辨率、灵敏度和定量方面持续提高其性能。本文综述了这种成像技术，特别强调了它的潜在用途和局限性、所需的仪器设备以及可能的成像几何结构和应用。详细介绍了主要的商用系统，并对技术发展的未来提出了一些展望。尽管体内小动物成像的绝大多数应用是基于落射照明平面成像，但该方法未来的成功在很大程度上依赖于基于与高空间分辨率结构模式（如MRI、CT或超声）结合使用的先进光学技术的新型成像系统的设计。
Fluorescence sampling of cellular function is widely used in all aspects of biology, allowing the visualization of cellular and sub-cellular biological processes with spatial resolutions in the range from nanometers up to centimeters. Imaging of fluorescence in vivo has become the most commonly used radiological tool in all pre-clinical work. In the last decade, full-body pre-clinical imaging systems have emerged with a wide range of utilities and niche application areas. The range of fluorescent probes that can be excited in the visible to near-infrared part of the electromagnetic spectrum continues to expand, with the most value for in vivo use being beyond the 630 nm wavelength, because the absorption of light sharply decreases. Whole-body in vivo fluorescence imaging has not yet reached a state of maturity that allows its routine use in the scope of large-scale pre-clinical studies. This is in part due to an incomplete understanding of what the actual fundamental capabilities and limitations of this imaging modality are. However, progress is continuously being made in research laboratories pushing the limits of the approach to consistently improve its performance in terms of spatial resolution, sensitivity and quantification. This paper reviews this imaging technology with a particular emphasis on its potential uses and limitations, the required instrumentation, and the possible imaging geometries and applications. A detailed account of the main commercially available systems is provided as well as some perspective relating to the future of the technology development. Although the vast majority of applications of in vivo small animal imaging are based on epi-illumination planar imaging, the future success of the method relies heavily on the design of novel imaging systems based on state-of-the-art optical technology used in conjunction with high spatial resolution structural modalities such as MRI, CT or ultra-sound.
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影响因子：
11.5
作者：
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通讯作者：
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通过近红外荧光叶酸靶向探针进行关节炎成像。
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作者：
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影响因子：
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作者：
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通讯作者：
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