Bovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth.
Bovine milk-derived exosomes from colostrum are enriched with proteins implicated in immune response and growth.
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DOI：
10.1038/s41598-017-06288-8
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发表时间：
2017-07-19
影响因子：
4.6
通讯作者：
Mathivanan S
中科院分区：
文献类型：
--
作者：
Samuel M;Chisanga D;Liem M;Keerthikumar S;Anand S;Ang CS;Adda CG;Versteegen E;Jois M;Mathivanan S
外泌体是多种细胞类型分泌到细胞外空间的细胞外囊泡。它们含有细胞状态特定的货物，通常反映细胞/生物体的（病理）生理状况。牛奶中含有大量的外泌体，目前尚不清楚它们的含量是否会根据生物体的哺乳阶段而改变。在这里，我们从哺乳不同阶段获得的牛乳中分离出外泌体，并通过定量蛋白质组学检查其含量。通过 OptiPrep 密度梯度离心从产犊后 24、48 和 72 小时后获得的奶牛中分离出外泌体。作为对照，还从泌乳中期的奶牛（称为成熟奶（MM））中分离出外泌体。外泌体的生化和生物物理特征揭示了初乳和 MM 样品中外泌体的丰度很高。定量蛋白质组学分析强调了基于奶牛泌乳状态的外泌体蛋白质组货物的变化。功能富集分析表明，初乳中的外泌体显着富含可能调节免疫反应和生长的蛋白质。这项研究强调了初乳中外泌体的重要性，从而为利用这些囊泡调节免疫反应和生长开辟了新途径。
Exosomes are extracellular vesicles secreted by multiple cell types into the extracellular space. They contain cell-state specific cargos which often reflects the (patho)physiological condition of the cells/organism. Milk contains high amounts of exosomes and it is unclear whether their cargo is altered based on the lactation stage of the organism. Here, we isolated exosomes from bovine milk that were obtained at various stages of lactation and examined the content by quantitative proteomics. Exosomes were isolated by OptiPrep density gradient centrifugation from milk obtained from cow after 24, 48 and 72 h post calving. As control, exosomes were also isolated from cows during mid-lactation period which has been referred to as mature milk (MM). Biochemical and biophysical characterization of exosomes revealed the high abundance of exosomes in colostrum and MM samples. Quantitative proteomics analysis highlighted the change in the proteomic cargo of exosomes based on the lactation state of the cow. Functional enrichment analysis revealed that exosomes from colostrum are significantly enriched with proteins that can potentially regulate the immune response and growth. This study highlights the importance of exosomes in colostrum and hence opens up new avenues to exploit these vesicles in the regulation of the immune response and growth.
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影响因子：
3.3
作者：
Reinhardt, Timothy A.;Lippolis, John D.;Sacco, Randy E.
通讯作者：
Sacco, Randy E.
DOI：
10.1631/jzus.2005.b1045
发表时间：
2005-11-01
期刊：
Journal of Zhejiang University. Science. B
影响因子：
--
作者：
Gruys, E;Toussaint, M J M;Koopmans, S J
通讯作者：
Koopmans, S J
影响因子：
3.4
作者：
Kalra, Hina;Adda, Christopher G.;Mathivanan, Suresh
通讯作者：
Mathivanan, Suresh
影响因子：
--
作者：
Gangoda L;Keerthikumar S;Fonseka P;Edgington LE;Ang CS;Ozcitti C;Bogyo M;Parker BS;Mathivanan S
通讯作者：
Mathivanan S
影响因子：
16
作者：
Pathan M;Keerthikumar S;Chisanga D;Alessandro R;Ang CS;Askenase P;Batagov AO;Benito-Martin A;Camussi G;Clayton A;Collino F;Di Vizio D;Falcon-Perez JM;Fonseca P;Fonseka P;Fontana S;Gho YS;Hendrix A;Hoen EN;Iraci N;Kastaniegaard K;Kislinger T;Kowal J;Kurochkin IV;Leonardi T;Liang Y;Llorente A;Lunavat TR;Maji S;Monteleone F;Øverbye A;Panaretakis T;Patel T;Peinado H;Pluchino S;Principe S;Ronquist G;Royo F;Sahoo S;Spinelli C;Stensballe A;Théry C;van Herwijnen MJC;Wauben M;Welton JL;Zhao K;Mathivanan S
通讯作者：
Mathivanan S