Identification of genes related to chlamydospore formation in Clonostachys rosea 67-1.
Identification of genes related to chlamydospore formation in Clonostachys rosea 67-1.
复制标题
红竹67-1厚垣孢子形成相关基因的鉴定
DOI：
10.1002/mbo3.624
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发表时间：
2019-01
期刊：
影响因子：
3.4
通讯作者：
Li SD
中科院分区：
文献类型：
--
作者：
Sun ZB;Zhang J;Sun MH;Li SD
厚壁孢子是真菌生物农药商品化的重要组成部分。为了探索与厚垣孢子形成相关的基因，对一株在特定条件下产生抗性孢子的生防菌Clonostachys rose67-1进行了转录组测序和分析。共获得549,661,174个清洁读数，并鉴定了一系列可能与真菌厚垣孢子形成有关的差异表达基因。在36小时，与分生孢子形成的对照相比，67和117个基因在厚垣孢子产生过程中上调和下调，53个和24个基因在72小时上调和下调。GO分类表明，差异表达基因与细胞成分、生物学过程和分子功能类别有关。通过KEGG分析，共有188条代谢途径与厚垣孢子的产生有关。用逆转录定量聚合酶链式反应验证了16个差异表达基因，其表达谱与转录组数据一致。据我们所知，这是关于沙棘厚垣孢子形成相关基因的首次报道。这些结果为深入了解海藻产孢量的分子机制提供了帮助，这将有助于真菌生防剂的开发。
Chlamydospores are specific structures that are of great significance to the commercialization of fungal biopesticides. To explore the genes associated with chlamydospore formation, a biocontrol fungus Clonostachys rosea 67‐1 that is capable of producing resistant spores under particular conditions was investigated by transcriptome sequencing and analysis. A total of 549,661,174 clean reads were obtained, and a series of differentially expressed genes potentially involved in fungal chlamydospore formation were identified. At 36 hr, 67 and 117 genes were up‐ and downregulated in C. rosea during chlamydospore production, compared with the control for conidiation, and 53 and 24 genes were up‐ and downregulated at 72 hr. GO classification suggested that the differentially expressed genes were related to cellular component, biological process, and molecular function categories. A total of 188 metabolism pathways were linked to chlamydospore production by KEGG analysis. Sixteen differentially expressed genes were verified by reverse transcription quantitative PCR, and the expression profiles were consistent with the transcriptome data. To the best of our knowledge, it is the first report on the genes associated with chlamydospore formation in C. rosea. The results provide insight into the molecular mechanisms underlying C. rosea sporulation, which will assist the development of fungal biocontrol agents.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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发表时间：
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