Serine/threonine protein kinase SpkG is a candidate for high salt resistance in the unicellular cyanobacterium Synechocystis sp. PCC 6803.
Serine/threonine protein kinase SpkG is a candidate for high salt resistance in the unicellular cyanobacterium Synechocystis sp. PCC 6803.
复制标题
丝氨酸/苏氨酸蛋白激酶 SpkG 是单细胞蓝藻集胞藻属高盐抗性的候选者。
DOI：
10.1371/journal.pone.0018718
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Shao HB
中科院分区：
文献类型：
--
作者：
Liang C;Zhang X;Chi X;Guan X;Li Y;Qin S;Shao HB
背景在单细胞蓝藻集胞藻PCC 6803中预测了7个丝氨酸/苏氨酸激酶基因。SpkA和SpkB被证明是细胞运动所必需的，SpkE没有激酶活性。目前还没有报道其他四种STK是否参与集胞藻PCC 6803中应激介导的信号传导。方法/主要发现在本文中，我们研究了其他四个丝氨酸/苏氨酸激酶，SpkC，SpkD，SpkF和SpkG的差异表达，在七个不同的压力条件。在高盐胁迫下，spkG基因转录水平上调，spkC基因转录水平下调。构建了两株spk缺失突变株ΔspkC和ΔspkG，并与野生株进行了比较。野生型菌株和ΔspkC突变体在高盐胁迫条件下不受影响。相反，spkG突变体的生长完全受损。为了进一步证实spkG的功能，我们还研究了spkG突变对盐胁迫诱导基因表达的影响。我们用DNA微阵列分析比较了野生型集胞藻PCC 6803和SpkG突变细胞之间的全基因组转录模式。结论本研究首次将spkG基因作为高盐信号的传感器进行了研究。我们认为，SpkG在集胞藻中直接感受高盐信号，而不是介导其他激酶的信号。本研究的基因芯片实验为进一步研究集胞藻PCC 6803在高盐胁迫下的信号转导机制提供了实验依据。
Background Seven serine/threonine kinase genes have been predicted in unicellular cyanobacterium Synechocystis sp. PCC6803. SpkA and SpkB were shown to be required for cell motility and SpkE has no kinase activity. There is no report whether the other four STKs are involved in stress-mediated signaling in Synechocystis PCC6803. Methodology/Principal Findings In this paper, we examined differential expression of the other four serine/threonine kinases, SpkC, SpkD, SpkF and SpkG, at seven different stress conditions. The transcriptional level was up-regulated of spkG and down-regulated of spkC under high salt stress condition. Two spk deletion mutants, ΔspkC and ΔspkG, were constructed and their growth characteristic were examined compared to the wild strain. The wild strain and ΔspkC mutant were not affected under high salt stress conditions. In contrast, growth of spkG mutant was completely impaired. To further confirm the function of spkG, we also examined the effect of mutation of spkG on the expression of salt stress-inducible genes. We compared genome-wide patterns of transcription between wild-type Synechocystis sp. PCC6803 and cells with a mutation in the SpkG with DNA microarray analysis. Conclusion In this study, we first study the spkG gene as sensor of high salt signal. We consider that SpkG play essential roles in Synechocystis sp. for sensing the high salt signal directly, rather than mediating signals among other kinases. Our microarray experiment may help select relatively significant genes for further research on mechanisms of signal transduction of Synechocystis sp. PCC6803 under high salt stress.
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DOI：
10.1006/bbrc.2001.6201
发表时间：
2002-01-11
影响因子：
3.1
作者：
Kanesaki, Y;Suzuki, I;Murata, N
通讯作者：
Murata, N
影响因子：
3.1
作者：
Echenique, J;Kadioglu, A;Trombe, MC
通讯作者：
Trombe, MC
影响因子：
3.6
作者：
Suzuki, I;Kanesaki, Y;Murata, N
通讯作者：
Murata, N
影响因子：
4.8
作者：
Shumskaya, MA;Paithoonrangsarid, K;Murata, N
通讯作者：
Murata, N
影响因子：
2.6
作者：
Kamei, A;Yoshihara, S;Ikeuchi, M
通讯作者：
Ikeuchi, M