Enhancing Fluorogold-based neural tract tracing.
Enhancing Fluorogold-based neural tract tracing.
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DOI：
10.1016/j.jneumeth.2016.06.004
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发表时间：
2016-09-01
影响因子：
3
通讯作者：
Howland, D. R.
中科院分区：
文献类型：
--
作者：
Mondello, S. E.;Jefferson, S. C.;O'Steen, W. A.;Howland, D. R.
Fluorogold (FG) is used by many groups to retrogradely trace nervous system pathways. Fluorogold, while a robust tracer, also is neurotoxic and causes tissue damage at the injection site and leads to motor deficits. In the current study, we describe a method for enhancing FG-uptake using Triton™ and an overall procedure for reducing FG-related tissue damage while still allowing effective quantification. Triton™ decreases the amount of FG, as well as the time required for long-distance transport from the thoracic spinal cord to the motor cortex by >4 fold when this distance is >10 in. Although small FG concentrations and injection volumes are ideal for minimizing associated tissue damage and motor deficits, they result in difficult-to-detect fluorescence. This can be solved using FG antiserum paired with an ABC chromogen reaction. This ABC chromogen reaction product can remain stable for at least 9 years. This study is the first to collectively address FG-induced tissue damage and describe methods for minimizing this damage. Triton™ enhances the uptake of FG in the nervous system, reduces the FG required, and allows for a substantial decrease in tracing time that limits FG-induced motor deficits. Small FG concentration and volume decreases tissue damage but also decreases FG fluorescent detection. Detection challenges are resolved using FG anti-serum and chromogen reactions.
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影响因子：
3.7
作者：
Falgairolle M;O'Donovan MJ
通讯作者：
O'Donovan MJ
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影响因子：
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作者：
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Puigdellívol-Sánchez, A;Valero-Cabré, A;Molander, C
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Molander, C
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MARANTO, AR
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作者：
BUHL, EH;DANN, JF
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