The T3S effector XopXccN of Xanthomonas campestris pv. campestris is involved in plant defense through interference with photosystems, reactive oxygen species (ROS) generation, and callose deposition
The T3S effector XopXccN of Xanthomonas campestris pv. campestris is involved in plant defense through interference with photosystems, reactive oxygen species (ROS) generation, and callose deposition
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Xanthomonas Campestris pv. 的 T3S 效应子 XopXccN。
DOI：
10.5897/ajmr12.149
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发表时间：
2012-04
影响因子：
--
通讯作者：
Tang, Dong-Jie
中科院分区：
文献类型：
--
作者：
Jiang, Guo-Feng;Lu, Guang-Tao;He, Yong-Qiang;Yu, Di-Qiu;Tang, Ji-Liang;Jiang, Bo-Le;Chen, Li-Gang;Liu, San;Wei, Hong-Yu;Cen, Wei-Jian;Hang, Xiao-Hong;Wen, Zong-Zhen;Tang, Dong-Jie
XopXccN和XopN分别是野油菜黄单胞菌野油菜致病变种（Xcc）和野油菜黄单胞菌水泡致病变种（Xcv）的重要III型分泌（T3 S）毒力效应蛋白。基于序列同源性，将它们置于相同的T3 S效应子类别中。已经证明Xcv XopN抑制Xcv感染期间病原体相关的分子模式触发的免疫应答。在这里，我们提出的证据表明，Xcv XopN不能取代XopXccN的毒力ofXcc。此外，我们还利用双向差异凝胶电泳技术研究了Xcc XopXccN对中国萝卜中差异表达蛋白的影响。结果表明，XopXccN抑制了9种主要与植物光系统相关的蛋白。RT-PCR分析表明，XopXccN在转录水平上抑制了几种蛋白的表达。此外，XopXccN还能抑制植物体内活性氧的产生和胼胝质的沉积。综上所述，我们的研究结果表明，XopXccN参与植物防御通过干扰光系统，ROS的产生，和胼胝质沉积在植物。
关键词：XopXccN，T3 S效应子，野油菜黄单胞菌野油菜致病变种，二维DIGE。
XopXccN and XopN are important type III secreted (T3S) virulence effector proteins ofXanthomonas campestris pv. campestris (Xcc) and Xanthomonas campestris pv.vesicatoria (Xcv), respectively. They were placed in the same T3S effector class based on sequence homology. It has been demonstrated that Xcv XopN suppresses pathogen-associated molecular pattern-triggered immune responses during Xcv infection. Here, we present evidence showing that Xcv XopN cannot replace XopXccN for the virulence ofXcc. In addition, we employed two-dimensional different gel electrophoresis to investigate the differentially expressed proteins affected by Xcc XopXccN in the host plant Chinese radish. The results demonstrated that nine proteins mainly associated with plant photosystems were suppressed by XopXccN. RT-PCR analysis showed that several proteins were repressed by XopXccN at the transcriptional level. Moreover, XopXccN could suppress the generation of reactive oxygen species (ROS) and callose deposition in plants. Taken together, our results revealed that XopXccN is involved in plant defense through interference with photosystems, ROS generation, and callose deposition in planta.
Key words: XopXccN, T3S effector, Xanthomonas campestris pv. campestris, 2D-DIGE.
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影响因子：
4.3
作者：
Jannat, Rayhanur;Uraji, Misugi;Morofuji, Miho;Islam, Mohammad Muzahidul;Bloom, Rachel E;Nakamura, Yoshimasa;McClung, C Robertson;Schroeder, Julian I;Mori, Izumi C;Murata, Yoshiyuki
通讯作者：
Murata, Yoshiyuki
影响因子：
7.4
作者：
Buettner, Daniela;He, Sheng Yang
通讯作者：
He, Sheng Yang
DOI：
10.1105/tpc.6.9.1301
发表时间：
1994-09
期刊：
The Plant cell
影响因子：
--
作者：
A. Price;A. Taylor;Scott J. Ripley;A. Griffiths;A. Trewavas;M. Knight
通讯作者：
A. Price;A. Taylor;Scott J. Ripley;A. Griffiths;A. Trewavas;M. Knight
影响因子：
14.8
作者：
Isaacson, Tal;Damasceno, Cynthia M. B.;Rose, Jocelyn K. C.
通讯作者：
Rose, Jocelyn K. C.
DOI：
--
发表时间：
2001-01
期刊：
--
影响因子：
--
作者：
J. Sambrook;E. Fritsch;T. Maniatis
通讯作者：
J. Sambrook;E. Fritsch;T. Maniatis