Arabidopsis CPR5 regulates ethylene signaling via molecular association with the ETR1 receptor.
Arabidopsis CPR5 regulates ethylene signaling via molecular association with the ETR1 receptor.
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DOI：
10.1111/jipb.12570
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发表时间：
2017-11
影响因子：
11.4
通讯作者：
Dong CH
中科院分区：
文献类型：
--
作者：
Wang F;Wang L;Qiao L;Chen J;Pappa MB;Pei H;Zhang T;Chang C;Dong CH
植物激素乙烯在植物生长发育和对环境胁迫的反应中起着多种作用。乙烯被膜结合的乙烯受体所感知，并且在拟南芥中的同源受体中，ETR 1乙烯受体起主要作用。本研究为拟南芥CPR 5作为一种新的ETR 1受体相互作用蛋白参与乙烯反应和信号转导提供了证据。酵母裂解泛素分析和植物细胞中的双荧光互补研究表明，CPR 5直接与ETR 1受体相互作用。遗传分析表明，cpr 5的突变等位基因可以抑制etr 1 -1和etr 1 -2的乙烯不敏感性，但不能抑制其他显性乙烯受体突变体。无论是在转基因拟南芥植株中，还是在烟草中异位表达拟南芥CPR 5，都显著增强了乙烯敏感性。这些发现表明CPR 5在调节乙烯信号传导中起着关键作用。CPR 5定位于内膜结构和细胞核，并参与各种调控途径，包括发病机制，叶片衰老和自发细胞死亡。本研究为CPR 5在拟南芥乙烯受体信号通路中发挥新的调控功能提供了证据。
The plant hormone ethylene plays various functions in plant growth, development and response to environmental stress. Ethylene is perceived by membrane-bound ethylene receptors, and among the homologous receptors in Arabidopsis, the ETR1 ethylene receptor plays a major role. The present study provides evidence demonstrating that Arabidopsis CPR5 functions as a novel ETR1 receptor-interacting protein in regulating ethylene response and signaling. Yeast split ubiquitin assays and bi-fluorescence complementation studies in plant cells indicated that CPR5 directly interacts with the ETR1 receptor. Genetic analyses indicated that mutant alleles of cpr5 can suppress ethylene insensitivity in both etr1-1 and etr1-2, but not in other dominant ethylene receptor mutants. Overexpression of Arabidopsis CPR5 either in transgenic Arabidopsis plants, or ectopically in tobacco, significantly enhanced ethylene sensitivity. These findings indicate that CPR5 plays a critical role in regulating ethylene signaling. CPR5 is localized to endomembrane structures and the nucleus, and is involved in various regulatory pathways, including pathogenesis, leaf senescence, and spontaneous cell death. This study provides evidence for a novel regulatory function played by CPR5 in the ethylene receptor signaling pathway in Arabidopsis.
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影响因子：
11.6
作者：
Hall, AE;Bleecker, AB
通讯作者：
Bleecker, AB
影响因子：
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作者：
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