Two orders of magnitude fluorescence enhancement of aluminum phthalocyanines by gold nanocubes: a remarkable improvement for cancer cell imaging and detection.
Two orders of magnitude fluorescence enhancement of aluminum phthalocyanines by gold nanocubes: a remarkable improvement for cancer cell imaging and detection.
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金纳米立方体对铝酞菁的荧光增强两个数量级：癌细胞成像和检测的显着改进。
DOI：
10.1021/am500106c
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发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Chen, Ji-Yao
中科院分区：
文献类型：
--
作者：
Xu, Yong-Kui;Hwang, Sekyu;Kim, Sungjee;Chen, Ji-Yao
金属纳米颗粒的金属增强荧光（MEF）是一种用于生物体系荧光检测的有效技术。研究了金纳米棒（AuNRs）和纳米立方体（AuNCs）对磺化酞菁铝（AlPcS）荧光增强的MEF效应。当AuNRs的长径比较低时，在600-750 nm范围内有相应的纵向表面等离子体共振（LSPR）带，其共轭AlPcS的荧光被猝灭，而当AuNRs的LSPR带在800-900 nm范围内时，其共轭AlPcS的MEF分别增强2-6倍。使用AuNCs，实现了AlPcS荧光的极大增强，增强因子为150倍。使用两种癌细胞系作为体外模型，在405 nm的单光子激发（OPE）下，相对于单独的AlPcS，获得了细胞中AlPcS-AuNCs缀合物的突出荧光增强。此外，在800 nm飞秒激光的双光子激发（TPE）下，细胞中的AlPcS-AuNCs也实现了明亮的荧光图像。使用OPE或TPE的高质量细胞成像证明了AlPcS-AuNC在癌细胞检测中的潜力。
The metal-enhanced fluorescence (MEF) by metal nanoparticles is a useful technique for fluorescence detections in biological systems. The MEF effects with gold nanorods (AuNRs) and nanocubes (AuNCs) for fluorescence enhancements of sulfonated aluminum phthalocyanine (AlPcS), a commonly used and clinical approved photosensitizer for photodynamic therapy of cancers, were studied in this work. For the AuNRs which have the low aspect ratios with the corresponding longitudinal surface plasma resonance (LSPR) band in the region of 600-750 nm, the fluorescence quenching of conjugated AlPcS was found. Whereas for the AuNRs that have the LSPR bands of 800-900 nm, the MEF of AlPcS was obtained with the enhancing factor of 2-6 times, respectively. Using AuNCs, a great enhancement of AlPcS fluorescence was achieved with an enhancing factor of 150 times. Using two cancer cell lines as in vitro models, an outstanding fluorescence enhancement of AlPcS-AuNCs conjugates in cells, relative to AlPcS alone, was obtained under one-photon excitation (OPE) of 405 nm. Moreover, the bright fluorescence image of AlPcS-AuNCs in cells was also achieved under the two-photon excitation (TPE) of an 800 nm femtosecond laser. The high-quality cell imaging with either OPE or TPE demonstrated the potential of AlPcS-AuNCs in cancer cell detections.
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