Variation in the fruit development gene POINTED TIP regulates protuberance of tomato fruit tip.

Variation in the fruit development gene POINTED TIP regulates protuberance of tomato fruit tip.
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果实发育基因 POINTED TIP 的变异调节番茄果尖的突起

DOI:
10.1038/s41467-022-33648-4
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发表时间:
2022-10-08
影响因子:
16.6
通讯作者:
--
中科院分区:
综合性期刊1区
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番茄的驯化导致了果实形态的显著变化。在这里,我们展示了一个全基因组关联研究(GWAS),以了解果尖的发展,并描述了一个POINTED TIP(PT)基因编码的C2H2型锌指转录因子。在PT基因C_2H_2结构域中发现了一个单核苷酸多态性,将组氨酸(H)变为精氨酸(R),这两个等位基因被称为PTH和PTR。敲除PTH基因可诱导尖果发育,PTH通过下调FUL2基因的转录,改变生长素的运输,从而抑制尖果的形成。我们的进化分析和其他人的先前研究表明,PTR等位基因可能在驯化过程中与其他选择的位点一起沿着。本研究揭示了番茄果尖发育的PT变异及其分子机制。
The domestication of tomato has led to striking variations in fruit morphology. Here, we show a genome-wide association study (GWAS) to understand the development of the fruit tip and describe aPOINTED TIP(PT) gene that encodes a C2H2-type zinc finger transcription factor. A single nucleotide polymorphism is found to change a histidine (H) to an arginine (R) in the C2H2 domain of PT and the two alleles are referred to asPTHandPTR. Knocking outPTHleads to development of pointed tip fruit.PTHfunctions to suppress pointed tip formation by downregulating the transcription ofFRUTFULL 2(FUL2), which alters the auxin transport. Our evolutionary analysis and previous studies by others suggest that thePTRallele likely hitch-hiked along with other selected loci during the domestication process. This study uncovers variation inPTand molecular mechanism underlying fruit tip development in tomato.
玉米不确定的1开花时间调节剂定义了高等植物中高度保守的锌指蛋白家族。
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