Impact of ageing and a synbiotic on the immune response to seasonal influenza vaccination; a randomised controlled trial.
Impact of ageing and a synbiotic on the immune response to seasonal influenza vaccination; a randomised controlled trial.
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DOI：
10.1016/j.clnu.2017.01.011
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发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Yaqoob P
中科院分区：
文献类型：
--
作者：
Enani S;Przemska-Kosicka A;Childs CE;Maidens C;Dong H;Conterno L;Tuohy K;Todd S;Gosney M;Yaqoob P
老龄化增加了呼吸道感染的风险，并削弱了对流感疫苗接种的反应。益生菌和益生菌提供了一个机会，通过改变肠道微生物群来调节抗病毒防御和对疫苗接种的反应。研究了一种新型益生菌长双歧杆菌Bifidobacterium longum bv. Cucug 52486与益生元葡萄糖低聚糖联合使用对年轻和老年受试者季节性流感疫苗接种的B和T细胞应答的影响。在一项双盲随机对照试验中，58名年轻（18-35岁）和54名老年（60-85岁）受试者补充合生元8周。4周后，他们接种了季节性流感疫苗。在基线、第4、6和8周评估B和T细胞表型以及对疫苗体外再刺激的反应性。B和T细胞谱在年轻和老年受试者之间存在显著差异。接种疫苗增加了年轻受试者的记忆、伊加+记忆、IgG+记忆和总IgG+ B细胞的数量，但在老年受试者中未能做到这一点，并且没有显著改变T细胞亚群。在老年受试者中，H1N1亚单位的血清转化与较高的疫苗接种后血浆B细胞数量相关，但血清转化与T细胞表型的相关性较低。年轻和老年受试者的B和T细胞亚群表现出强烈的抗原特异性回忆激发，尽管不受年龄影响，但对回忆激发的反应性与血清转化相关。在老年受试者中，CMV血清阳性与对疫苗的回忆反应显著降低相关，但合生元不影响B或T细胞对流感疫苗再刺激的反应性。抗原特异性B和T细胞激活后，在体外召回的挑战与流感疫苗的CMV血清阳性的影响，但不是由合生素。在ClinicalTrials.gov下注册，标识号NCT 01066377。
Ageing increases risk of respiratory infections and impairs the response to influenza vaccination. Pre- and pro-biotics offer an opportunity to modulate anti-viral defenses and the response to vaccination via alteration of the gut microbiota. This study investigated the effect of a novel probiotic, Bifidobacterium longum bv. infantis CCUG 52486, combined with a prebiotic, gluco-oligosaccharide, on the B and T cell response to seasonal influenza vaccination in young and older subjects . In a double-blind, randomized controlled trial, 58 young (18–35 y) and 54 older (60–85 y) subjects were supplemented with the synbiotic for 8 weeks. At 4 weeks they were administered with a seasonal influenza vaccine. B and T cell phenotype and responsiveness to in vitro re-stimulation with the vaccine were assessed at baseline, 4, 6 and 8 weeks. B and T cell profiles differed markedly between young and older subjects. Vaccination increased numbers of memory, IgA+ memory, IgG+ memory and total IgG+ B cells in young subjects, but failed to do so in older subjects and did not significantly alter T cell subsets. Seroconversion to the H1N1 subunit in the older subjects was associated with higher post-vaccination numbers of plasma B cells, but seroconversion was less consistently associated with T cell phenotype. B and T cell subsets from both young and older subjects demonstrated a strong antigen-specific recall challenge, and although not influenced by age, responsiveness to the recall challenge was associated with seroconversion. In older subjects, CMV seropositivity was associated with a significantly lower recall response to the vaccine, but the synbiotic did not affect the responsiveness of B or T cells to re-stimulation with influenza vaccine. Antigen-specific B and T cell activation following an in vitro recall challenge with the influenza vaccine was influenced by CMV seropositivity, but not by a synbiotic. Registered under ClinicalTrials.gov Identifier no. NCT01066377.
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DOI：
10.1186/s12979-016-0061-4
发表时间：
2016
期刊：
Immunity & ageing : I & A
影响因子：
--
作者：
Przemska-Kosicka A;Childs CE;Enani S;Maidens C;Dong H;Dayel IB;Tuohy K;Todd S;Gosney MA;Yaqoob P
通讯作者：
Yaqoob P
影响因子：
120.7
作者：
Thompson, WW;Shay, DK;Fukuda, K
通讯作者：
Fukuda, K
影响因子：
4.4
作者：
Derhovanessian, Evelyna;Maier, Andrea B.;Pawelec, Graham
通讯作者：
Pawelec, Graham
影响因子：
4.4
作者：
Frasca, Daniela;Diaz, Alain;Blomberg, Bonnie B.
通讯作者：
Blomberg, Bonnie B.
影响因子：
7.2
作者：
Hudon, C;Fortin, A;Vanasse, A
通讯作者：
Vanasse, A