Differences in cecal microbiome of selected high and low feather-pecking laying hens.
Differences in cecal microbiome of selected high and low feather-pecking laying hens.
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DOI：
10.3382/ps/pey167
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发表时间：
2018-09-01
期刊：
影响因子：
4.4
通讯作者：
Harlander-Matauschek A
中科院分区：
文献类型：
--
作者：
Birkl P;Bharwani A;Kjaer JB;Kunze W;McBride P;Forsythe P;Harlander-Matauschek A
在哺乳动物中，越来越清楚的是，肠道微生物群不仅影响胃肠道生理，而且调节行为。在家禽中，盲肠有最多的胃肠道微生物群。蛋鸡啄羽毛行为是现代农业中尚未解决的重要行为问题之一。本研究的目的是评估60周龄时不同选择的高（HFP, n = 20）和低（LFP, n = 20）啄羽鸟类盲肠微生物群落。盲肠样本在MiSeq Illumina测序仪上使用改进的条形码Illumina测序方法进行16S rRNA群落分析和硅宏基因组学分析。结果表明，与HFP鸟类相比，LFP鸟类的总体微生物多样性（β多样性）有所增加，布雷-柯蒂斯指数差异（R2 = 0.171, P < 0.05）。此外，操作分类单位比较显示，与LFP鸟类相比，HFP鸟类中Clostridiae的存在增加，乳酸杆菌的存在减少（假发现率< 0.05，Mann-Whitney比较）。我们的数据表明，这两种蛋鸡的盲肠轮廓可能存在差异。在第一次研究的基础上，需要更多的研究，利用测序技术来分析鸡盲肠微生物群，以揭示FP的性能和盲肠微生物群落之间是否存在以及如何存在功能联系。
In mammals, it has become increasingly clear that the gut microbiota influences not only gastrointestinal physiology but also modulates behavior. In domestic birds, ceca have the greatest gastrointestinal microbial population. Feather-pecking (FP) behavior in laying hens is one of the most important unsolved behavioral issues in modern agriculture. The aim of the present study was to assess the cecal microbial community of divergently selected high (HFP; n = 20) and low (LFP; n = 20) feather-pecking birds at 60 wk of age. The cecal samples were subjected to community profiling of 16S rRNA and in silico metagenomics using a modified bar-coded Illumina sequencing method on a MiSeq Illumina sequencer. Our results revealed that compared to HFP birds, LFP birds are characterized by an increased overall microbial diversity (beta diversity) shown by a difference in the Bray–Curtis index (R2 = 0.171, P < 0.05). Furthermore, operational taxonomic unit comparisons showed an increased presence of Clostridiae and decreased presence of Lactobaccillacae in HFP birds when compared to LFP birds (False Discovery Rate < 0.05, Mann–Whitney comparisons). Our data indicate that there may be differences in the cecal profile between these 2 lines of laying hens. More research, building on this first study using sequencing technology for profiling the chicken cecal microbiome, will be needed in order to reveal if and how there exists a functional link between the performance of FP and the cecal microbial community.
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影响因子：
4.4
作者：
Lovanh, N.;Cook, K. L.;Sistani, K.
通讯作者：
Sistani, K.
DOI：
10.1126/science.1198719
发表时间：
2011-05-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Muegge BD;Kuczynski J;Knights D;Clemente JC;González A;Fontana L;Henrissat B;Knight R;Gordon JI
通讯作者：
Gordon JI
影响因子：
4.4
作者：
Lu, JR;Idris, U;Lee, MD
通讯作者：
Lee, MD
影响因子：
4.4
作者：
Bartram, Andrea K.;Lynch, Michael D. J.;Neufeld, Josh D.
通讯作者：
Neufeld, Josh D.
影响因子：
2.3
作者：
McKeegan, DEF;Savory, CJ
通讯作者：
Savory, CJ