Comparing thoracic and intra-nasal pressure transients to monitor active odor sampling during odor-guided decision making in the mouse.
Comparing thoracic and intra-nasal pressure transients to monitor active odor sampling during odor-guided decision making in the mouse.
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DOI：
10.1016/j.jneumeth.2013.09.006
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发表时间：
2014-01-15
影响因子：
3
通讯作者：
Gelperin, Alan
中科院分区：
文献类型：
--
作者：
Reisert, Johannes;Golden, Glen J.;Matsumura, Koichi;Smear, Matt;Rinberg, Dmitry;Gelperin, Alan
近年来，例如由于记录设备的小型化程度提高，对行为小鼠生理参数的记录有了极大的增长。对于气味驱动的行为而言，一个特别重要的参数是呼吸频率，因为呼吸频率决定了气味输送到鼻腔以及位于鼻腔内的嗅觉受体神经元的速率。
通常，呼吸模式是通过测量鼻腔内呼吸引起的温度或压力变化来监测的。这两种方法都需要植入鼻插管，并将小鼠用电缆或管子拴住。为了避免这些局限性，我们使用了一种植入式压力传感器，它可以读取胸腔压力并远程传输数据，因此适用于需要动物自由活动的实验。
小鼠在植入压力传感器的情况下进行了一个气味驱动的“做/不做”行为任务，事实证明该传感器能可靠地工作，从而可以在数周内对某只动物的呼吸信号进行记录。
我们同时记录了胸腔和鼻腔的压力变化，发现测量胸腔压力变化与测量鼻腔压力变化得到的结果相似。
远程记录的呼吸信号是一种监测气味引导的呼吸频率行为变化的可行方法。当对自由活动的动物进行数周的记录时，其优势最为显著。文中还讨论了记录呼吸模式的不同方法的优缺点。
Recording of physiological parameters in behaving mice has seen an immense increase over recent years driven by, for example, increased miniaturization of recording devices. One parameter particularly important for odorant-driven behaviors is the breathing frequency, since the latter dictates the rate of odorant delivery to the nasal cavity and the olfactory receptor neurons located therein. Typically, breathing patterns are monitored by either measuring the breathing-induced temperature or pressure changes in the nasal cavity. Both require the implantation of a nasal cannula and tethering of the mouse to either a cable or tubing. To avoid these limitations we used an implanted pressure sensor which reads the thoracic pressure and transmits the data telemetrically, thus making it suitable for experiments which require a freely moving animal. Mice performed a Go/NoGo odorant-driven behavioral task with the implanted pressure sensor, which proved to work reliably to allow recording of breathing signals over several weeks from a given animal. We simultaneously recorded the thoracic and nasal pressure changes and found that measuring the thoracic pressure change yielded similar results compared to measurements of nasal pressure changes. Telemetrically recorded breathing signals are a feasible method to monitor odorant-guided behavioral changes in breathing rates. Its advantages are most significant when recording from a freely moving animal over several weeks. The advantages and disadvantages of different methods to record breathing patterns are discussed.
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影响因子：
2
作者：
Sela, Lee;Sobel, Noam
通讯作者：
Sobel, Noam
DOI：
10.1523/jneurosci.4101-12.2013
发表时间：
2013-01-02
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Cenier T;McGann JP;Tsuno Y;Verhagen JV;Wachowiak M
通讯作者：
Wachowiak M
DOI：
10.1523/jneurosci.4278-11.2012
发表时间：
2012-01-04
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Phillips ME;Sachdev RN;Willhite DC;Shepherd GM
通讯作者：
Shepherd GM
影响因子：
2.5
作者：
Courtiol, Emmanuelle;Hegoburu, Chloe;Buonviso, Nathalie
通讯作者：
Buonviso, Nathalie
影响因子：
25
作者：
Dombeck, Daniel A.;Harvey, Christopher D.;Tian, Lin;Looger, Loren L.;Tank, David W.
通讯作者：
Tank, David W.